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Literature summary extracted from

  • Kubota, T.; Matsushita, H.; Tomita, T.; Kosono, S.; Yoshida, M.; Kuzuyama, T.; Nishiyama, M.
    Novel stand-alone RAM domain protein-mediated catalytic control of anthranilate phosphoribosyltransferase in tryptophan biosynthesis in Thermus thermophilus (2017), Extremophiles, 21, 73-83 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.18 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.18 additional information enzyme activity is not affected by the sole addition of SraA, Trp, Ile, Leu or Ser Thermus thermophilus
2.4.2.18 tryptophan the enzyme is inhibited by tryptophan only in the presence of increasing amounts of stand-alone RAM domain protein SraA. Tryptophan acts as a specific inhibitor of the enzyme by binding to SraA Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
 N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18 Strep-Tactin column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.18 ? x * 36000, His-tagged enzyme, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 AnPRT
-
 Thermus thermophilus
2.4.2.18 TTC1491
-
 Thermus thermophilus