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Literature summary extracted from

  • Fessner, W.D.; Mertsch, A.; He, N.; Yi, D.; Kickstein, M.
    An alpha2,3-sialyltransferase from Photobacterium phosphoreum with broad substrate scope Controlling hydrolytic activity by directed evolution (2020), Chem. Eur. J., 26, 11614-11624 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.4 expressed in Escherichia coli BL21(DE3) cells Photobacterium phosphoreum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.4 A151D the mutant shows significantly improved properties as compared to the native enzyme. The mutant displays overall best performance in the synthesis of the GM3 trisaccharide (alpha2,3-Neu5Ac-Lac) from lactose in a one-pot, two-enzyme cascade Photobacterium phosphoreum
2.4.3.4 L387A the mutant shows significantly improved properties as compared to the native enzyme Photobacterium phosphoreum
2.4.3.4 S359T/S360T the mutant shows significantly improved properties as compared to the native enzyme Photobacterium phosphoreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.6 CMP-N-acetyl-beta-neuraminate + Lewis X Homo sapiens
-
 CMP + sialyl Lewis X
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.4 Photobacterium phosphoreum A5LHX0
-
-
2.4.3.4 Photobacterium phosphoreum JT-ISH-467 A5LHX0
-
-
2.4.3.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.4 Ni-affinity column chromatography Photobacterium phosphoreum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.6 RL95-2 cell
-
 Homo sapiens
-
2.4.3.6 uterine endometrium
-
 Homo sapiens
-
2.4.3.6 uterus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.4 CMP-N-acetyl-beta-neuraminate + lactose
-
 Photobacterium phosphoreum CMP + alpha2,3-SiaLac + Neu5Ac
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + lactose
-
 Photobacterium phosphoreum JT-ISH-467 CMP + alpha2,3-SiaLac + Neu5Ac
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + Lewis X
-
 Homo sapiens CMP + sialyl Lewis X
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.6 ? x * 48000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.3.4 2,3SiaT
-
 Photobacterium phosphoreum
2.4.3.6 beta-galactoside-a2,3-sialyltransferase III
-
 Homo sapiens
2.4.3.6 ST3Gal3
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.3.4 47.1
-
 melting temperature Photobacterium phosphoreum

Expression

EC Number Organism Comment Expression
2.4.3.6 Homo sapiens human endometrial tissues at secretory phase express a 4fold higher enzyme level relative to the tissues at proliferative phase up

General Information

EC Number General Information Comment Organism
2.4.3.6 malfunction enzyme downregulation significantly impairs the receptive ability of human endometrial RL95-2 cells to trophoblastic cells in vitro and inhibited implantation in pregnant mice Homo sapiens
2.4.3.6 metabolism ST3Gal3 is the key enzyme responsible for sialyl Lewis X oligosaccharide biosynthesis Homo sapiens
2.4.3.6 physiological function the enzyme facilitates endometrial receptivity through increasing sLeX oligosaccharide Homo sapiens