BRENDA - Enzyme Database

Long-chain acyl-CoA synthetase I and II from Candida lipolytica

Hosaka, K.; Mishina, M.; Kamiryo, T.; Numa, S.; Methods Enzymol. 71, 325-333 (1981)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.2.1.3
phosphatidylcholine
absolute requirement, acyl-CoA synthetase II
Yarrowia lipolytica
6.2.1.3
phosphatidylethanolamine
slight stimulation, acyl-CoA synthetase II
Yarrowia lipolytica
6.2.1.3
phosphatidylserine
slight stimulation, acyl-CoA synthetase II
Yarrowia lipolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.3
0.042
-
CoA
-
Yarrowia lipolytica
6.2.1.3
0.11
-
dephospho-CoA
-
Yarrowia lipolytica
6.2.1.3
0.5
-
ATP
N6-etheno-CoA
Yarrowia lipolytica
6.2.1.3
2.5
-
pantetheine
-
Yarrowia lipolytica
6.2.1.3
3.3
-
4'-phosphopantetheine
-
Yarrowia lipolytica
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.3
microbody
enzyme form acyl-CoA synthetase II
Yarrowia lipolytica
42579
-
6.2.1.3
microsome
enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
-
-
6.2.1.3
mitochondrion
enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.3
84000
-
x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.3
ATP + long-chain carboxylic acid + CoA
Yarrowia lipolytica
acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.3
Yarrowia lipolytica
-
2 enzyme forms: long-chain acyl-CoA synthetase I and long-chain acyl-CoA synthetase II
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.3
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.2.1.3
10.8
-
-
Yarrowia lipolytica
Storage Stability
EC Number
Storage Stability
Organism
6.2.1.3
-70░C, stable for at least 1 month
Yarrowia lipolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.3
ATP + decanoate + CoA
ineffective
759
Yarrowia lipolytica
AMP + diphosphate + decanoyl-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + 4'-phosphopantetheine
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-4'-phosphopantetheine
-
-
-
-
6.2.1.3
ATP + fatty acid + dephospho-CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-dephospho-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + N6-etheno-CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-N6-etheno-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + pantetheine
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-pantetheine
-
-
-
-
6.2.1.3
ATP + long-chain carboxylic acid + CoA
utilizes straight-chain fatty acids with 14-18 carbon atoms regardless of the degree of unsaturation. Straight-chain fatty acids containing more than 18 or fewer than 14 carbon atoms, as well as 16-hydroxypalmitic acid and hexadecanedioic acid are ineffective. Acyl-CoA synthetase II has a broader fatty acid specificity than acyl-CoA synthetase I. The effective substrates include long-chain dicarboxylic acids
759
Yarrowia lipolytica
AMP + diphosphate + long-chain acyl-CoA
-
-
-
-
6.2.1.3
ATP + long-chain carboxylic acid + CoA
acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation
759
Yarrowia lipolytica
?
-
-
-
-
6.2.1.3
ATP + octanoate + CoA
ineffective
759
Yarrowia lipolytica
AMP + diphosphate + octanoyl-CoA
-
-
-
-
6.2.1.3
ATP + oleate + CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + oleoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitate + CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + palmitoyl-CoA
-
-
-
-
6.2.1.3
dATP + fatty acid + CoA
-
759
Yarrowia lipolytica
dAMP + diphosphate + acyl-CoA
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.2.1.3
?
x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.3
7.1
9.6
-
Yarrowia lipolytica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.2.1.3
phosphatidylcholine
absolute requirement, acyl-CoA synthetase II
Yarrowia lipolytica
6.2.1.3
phosphatidylethanolamine
slight stimulation, acyl-CoA synthetase II
Yarrowia lipolytica
6.2.1.3
phosphatidylserine
slight stimulation, acyl-CoA synthetase II
Yarrowia lipolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.3
0.042
-
CoA
-
Yarrowia lipolytica
6.2.1.3
0.11
-
dephospho-CoA
-
Yarrowia lipolytica
6.2.1.3
0.5
-
ATP
N6-etheno-CoA
Yarrowia lipolytica
6.2.1.3
2.5
-
pantetheine
-
Yarrowia lipolytica
6.2.1.3
3.3
-
4'-phosphopantetheine
-
Yarrowia lipolytica
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.3
microbody
enzyme form acyl-CoA synthetase II
Yarrowia lipolytica
42579
-
6.2.1.3
microsome
enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
-
-
6.2.1.3
mitochondrion
enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.3
84000
-
x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.3
ATP + long-chain carboxylic acid + CoA
Yarrowia lipolytica
acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.3
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.2.1.3
10.8
-
-
Yarrowia lipolytica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.2.1.3
-70░C, stable for at least 1 month
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.3
ATP + decanoate + CoA
ineffective
759
Yarrowia lipolytica
AMP + diphosphate + decanoyl-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + 4'-phosphopantetheine
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-4'-phosphopantetheine
-
-
-
-
6.2.1.3
ATP + fatty acid + dephospho-CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-dephospho-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + N6-etheno-CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-N6-etheno-CoA
-
-
-
-
6.2.1.3
ATP + fatty acid + pantetheine
-
759
Yarrowia lipolytica
AMP + diphosphate + acyl-pantetheine
-
-
-
-
6.2.1.3
ATP + long-chain carboxylic acid + CoA
utilizes straight-chain fatty acids with 14-18 carbon atoms regardless of the degree of unsaturation. Straight-chain fatty acids containing more than 18 or fewer than 14 carbon atoms, as well as 16-hydroxypalmitic acid and hexadecanedioic acid are ineffective. Acyl-CoA synthetase II has a broader fatty acid specificity than acyl-CoA synthetase I. The effective substrates include long-chain dicarboxylic acids
759
Yarrowia lipolytica
AMP + diphosphate + long-chain acyl-CoA
-
-
-
-
6.2.1.3
ATP + long-chain carboxylic acid + CoA
acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation
759
Yarrowia lipolytica
?
-
-
-
-
6.2.1.3
ATP + octanoate + CoA
ineffective
759
Yarrowia lipolytica
AMP + diphosphate + octanoyl-CoA
-
-
-
-
6.2.1.3
ATP + oleate + CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + oleoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitate + CoA
-
759
Yarrowia lipolytica
AMP + diphosphate + palmitoyl-CoA
-
-
-
-
6.2.1.3
dATP + fatty acid + CoA
-
759
Yarrowia lipolytica
dAMP + diphosphate + acyl-CoA
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.2.1.3
?
x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I
Yarrowia lipolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.3
7.1
9.6
-
Yarrowia lipolytica