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Literature summary extracted from

  • Hosaka, K.; Mishina, M.; Kamiryo, T.; Numa, S.
    Long-chain acyl-CoA synthetase I and II from Candida lipolytica (1981), Methods Enzymol., 71, 325-333.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.3 phosphatidylcholine absolute requirement, acyl-CoA synthetase II Yarrowia lipolytica
6.2.1.3 phosphatidylethanolamine slight stimulation, acyl-CoA synthetase II Yarrowia lipolytica
6.2.1.3 phosphatidylserine slight stimulation, acyl-CoA synthetase II Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.042
-
 CoA
-
 Yarrowia lipolytica
6.2.1.3 0.11
-
 dephospho-CoA
-
 Yarrowia lipolytica
6.2.1.3 0.5
-
 ATP N6-etheno-CoA Yarrowia lipolytica
6.2.1.3 2.5
-
 pantetheine
-
 Yarrowia lipolytica
6.2.1.3 3.3
-
 4'-phosphopantetheine
-
 Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 microbody enzyme form acyl-CoA synthetase II Yarrowia lipolytica 42579
-
6.2.1.3 microsome enzyme form acyl-CoA synthetase I Yarrowia lipolytica
-
-
6.2.1.3 mitochondrion enzyme form acyl-CoA synthetase I Yarrowia lipolytica 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.3 84000
-
 x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 ATP + long-chain carboxylic acid + CoA Yarrowia lipolytica acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Yarrowia lipolytica
-
 2 enzyme forms: long-chain acyl-CoA synthetase I and long-chain acyl-CoA synthetase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3
-
 Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 10.8
-
-
 Yarrowia lipolytica

Storage Stability

EC Number Storage Stability Organism
6.2.1.3 -70°C, stable for at least 1 month Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + decanoate + CoA ineffective Yarrowia lipolytica AMP + diphosphate + decanoyl-CoA
-
 ?
6.2.1.3 ATP + fatty acid + 4'-phosphopantetheine
-
 Yarrowia lipolytica AMP + diphosphate + acyl-4'-phosphopantetheine
-
 ?
6.2.1.3 ATP + fatty acid + dephospho-CoA
-
 Yarrowia lipolytica AMP + diphosphate + acyl-dephospho-CoA
-
 ?
6.2.1.3 ATP + fatty acid + N6-etheno-CoA
-
 Yarrowia lipolytica AMP + diphosphate + acyl-N6-etheno-CoA
-
 ?
6.2.1.3 ATP + fatty acid + pantetheine
-
 Yarrowia lipolytica AMP + diphosphate + acyl-pantetheine
-
 ?
6.2.1.3 ATP + long-chain carboxylic acid + CoA utilizes straight-chain fatty acids with 14-18 carbon atoms regardless of the degree of unsaturation. Straight-chain fatty acids containing more than 18 or fewer than 14 carbon atoms, as well as 16-hydroxypalmitic acid and hexadecanedioic acid are ineffective. Acyl-CoA synthetase II has a broader fatty acid specificity than acyl-CoA synthetase I. The effective substrates include long-chain dicarboxylic acids Yarrowia lipolytica AMP + diphosphate + long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + long-chain carboxylic acid + CoA acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation Yarrowia lipolytica ?
-
 ?
6.2.1.3 ATP + octanoate + CoA ineffective Yarrowia lipolytica AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA
-
 Yarrowia lipolytica AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA
-
 Yarrowia lipolytica AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 dATP + fatty acid + CoA
-
 Yarrowia lipolytica dAMP + diphosphate + acyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 ? x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.3 7.1 9.6
-
 Yarrowia lipolytica