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Literature summary extracted from

  • Pawelczyk, T.; Angielski, S.
    Effect of ionic strength on the regulatory properties of 2-oxoglutarate dehydrogenase complex (1992), Biochimie, 74, 171-176 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.105 ADP the Sa0.5 for ADP in the absence of Ca2+ decreases as ionic strength increases. In the presence of calcium and 0.2 M ionic strength, ADP has no effect on 2-oxoglutarate dehydrogenase complex activity Sus scrofa
1.2.1.105 ATP the magnitude of inhibition by ATP is not influenced by changes in ionic strength in the absence of calcium. In the presence of Ca2+, increases in ionic strength lower the inhibitory effects of ATP. The S0.5 for ATP in both presence and absence of Ca2+ is not affected by changes in ionic strength in the range of 0.1-0.2 M Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.105 additional information
-
 additional information the Hill coefficient for 2-oxoglutarate and NAD at 0.2 M ionic strength is 1.0, whereas at 0.05 M ionic strength it is 0.85 and 1.2 for 2-oxoglutarate and NAD, respectively, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.0032
-
 CoA S0.5 value, 0.15 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.0035
-
 CoA S0.5 value, 0.10 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.0042
-
 CoA S0.5 value, 0.05 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.0042
-
 CoA S0.5 value, 0.20 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.031
-
 NAD+ S0.5 value, 0.05 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.038
-
 NAD+ S0.5 value, 0.10 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.05
-
 NAD+ S0.5 value, 0.15 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.06
-
 NAD+ S0.5 value, 0.20 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.16
-
 2-oxoglutarate S0.5 value, 0.20 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.27
-
 2-oxoglutarate S0.5 value, 0.15 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.43
-
 2-oxoglutarate S0.5 value, 0.10 M ionic strength, pH 7.8, 30°C Sus scrofa
1.2.1.105 0.65
-
 2-oxoglutarate S0.5 value, 0.05 M ionic strength, pH 7.8, 30°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.105 additional information the enzyme shows an optimum activity at 0.1 M ionic strength. Increase in ionic strength from 0.1 M to 0.2 M results in a decrease of S0.5 for 2-oxoglutarate, and in an increase of S0.5 for NAD. Changes in ionic strength over the range of 0.05-0.2 M have little, if any, effect on S0.5 for CoA Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.105 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.105 renal cortex
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.105 2-oxoglutarate + CoA + NAD+
-
 Sus scrofa succinyl-CoA + CO2 + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.105 7.4 7.6 at 0.05 M ionic strength Sus scrofa
1.2.1.105 7.8
-
 at 0.15 M ionic strength Sus scrofa