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Literature summary extracted from

  • Joshi, J.; Beaudoin, G.; Patterson, J.; García-García, J.; Belisle, C.; Chang, L.; Li, L.; Duncan, O.; Millar, A.; Hanson, A.
    Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s (2020), Biochem. J., 477, 2055-2069 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.60 expressed in Escherichia coli BL21(DE3) CodonPlus cells Triticum aestivum
2.4.2.60 expressed in Escherichia coli BL21(DE3) CodonPlus cells Hordeum vulgare
2.4.2.60 expressed in Escherichia coli BL21(DE3) CodonPlus cells Arabidopsis thaliana
2.4.2.60 expressed in Escherichia coli BL21(DE3) CodonPlus cells Avena sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.60 Fe2+ dependent on Triticum aestivum
2.4.2.60 Fe2+ dependent on Hordeum vulgare
2.4.2.60 Fe2+ dependent on Arabidopsis thaliana
2.4.2.60 Fe2+ dependent on Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Triticum aestivum
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Hordeum vulgare
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Arabidopsis thaliana
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Avena sativa
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.60 Arabidopsis thaliana
-
ecotype Col-0
-
2.4.2.60 Avena sativa
-
-
-
2.4.2.60 Hordeum vulgare
-
cultivar Morex
-
2.4.2.60 Triticum aestivum
-
cultivars Wyalkatchem and Chinese Spring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.60 Ni2+ affinity column chromatography Triticum aestivum
2.4.2.60 Ni2+ affinity column chromatography Hordeum vulgare
2.4.2.60 Ni2+ affinity column chromatography Arabidopsis thaliana
2.4.2.60 Ni2+ affinity column chromatography Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.60 leaf
-
Triticum aestivum
-
2.4.2.60 leaf
-
Hordeum vulgare
-
2.4.2.60 leaf
-
Arabidopsis thaliana
-
2.4.2.60 leaf
-
Avena sativa
-
2.4.2.60 root
-
Triticum aestivum
-
2.4.2.60 root
-
Hordeum vulgare
-
2.4.2.60 root
-
Arabidopsis thaliana
-
2.4.2.60 root
-
Avena sativa
-
2.4.2.60 seed enzyme without active-site Cys is the major enzyme type in developing grains Triticum aestivum
-
2.4.2.60 seed enzyme without active-site Cys is the major enzyme type in developing grains Hordeum vulgare
-
2.4.2.60 seed enzyme without active-site Cys is the major enzyme type in developing grains Arabidopsis thaliana
-
2.4.2.60 seed enzyme without active-site Cys is the major enzyme type in developing grains Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Triticum aestivum nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Hordeum vulgare nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Arabidopsis thaliana nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Avena sativa nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.60 Thi4
-
Triticum aestivum
2.4.2.60 Thi4
-
Hordeum vulgare
2.4.2.60 Thi4
-
Arabidopsis thaliana
2.4.2.60 Thi4
-
Avena sativa

General Information

EC Number General Information Comment Organism
2.4.2.60 metabolism the enzyme synthesizes the thiazole precursor of thiamin Triticum aestivum
2.4.2.60 metabolism the enzyme synthesizes the thiazole precursor of thiamin Hordeum vulgare
2.4.2.60 metabolism the enzyme synthesizes the thiazole precursor of thiamin Arabidopsis thaliana
2.4.2.60 metabolism the enzyme synthesizes the thiazole precursor of thiamin Avena sativa
2.4.2.60 physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Triticum aestivum
2.4.2.60 physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Hordeum vulgare
2.4.2.60 physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Arabidopsis thaliana
2.4.2.60 physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Avena sativa