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Literature summary extracted from

  • Del Arco, J.; Martinez, M.; Donday, M.; Clemente-Suarez, V.; Fernandez-Lucas, J.
    Cloning, expression and biochemical characterization of xanthine and adenine phosphoribosyltransferases from Thermus thermophilus HB8 (2018), Biocatal. Biotransform., 36, 216-223 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 gene aprt2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Thermus thermophilus
2.4.2.22 gene xprt, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+ required Thermus thermophilus
2.4.2.22 Mg2+ required Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 additional information
-
analytical ultracentrifugation analysis Thermus thermophilus
2.4.2.22 additional information
-
analytical ultracentrifugation analysis Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
AMP + diphosphate
-
r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 579
-
AMP + diphosphate
-
r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC 27634
-
AMP + diphosphate
-
r
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
XMP + diphosphate
-
r
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 579
-
XMP + diphosphate
-
r
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC 27634
-
XMP + diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Thermus thermophilus Q5SHW6
-
-
2.4.2.7 Thermus thermophilus ATCC 27634 Q5SHW6
-
-
2.4.2.7 Thermus thermophilus DSM 579 Q5SHW6
-
-
2.4.2.22 Thermus thermophilus Q5SKV8
-
-
2.4.2.22 Thermus thermophilus ATCC 27634 Q5SKV8
-
-
2.4.2.22 Thermus thermophilus DSM 579 Q5SKV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by heat treatement, nickel affinity chromatography, and gel filtration Thermus thermophilus
2.4.2.22 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by heat treatement, nickel affinity chromatography, and gel filtration Thermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 commercial preparation
-
Thermus thermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 0.32
-
purified recombinant enzyme, pH 8.5, 70°C, substrate adenine Thermus thermophilus
2.4.2.22 0.1
-
purified recombinant enzyme, pH 6.0, 70°C, substrate guanine Thermus thermophilus
2.4.2.22 0.12
-
purified recombinant enzyme, pH 6.0, 70°C, substrate hypoxanthine Thermus thermophilus
2.4.2.22 1.7
-
purified recombinant enzyme, pH 6.0, 70°C, substrate xanthine Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
2.4.2.7 -20--80°C, purified recombinant His-tagged enzyme, 70-80% activity reamining after 70 days Thermus thermophilus
2.4.2.7 4°C, purified recombinant His-tagged enzyme, 85% activity remaining after 70 days Thermus thermophilus
2.4.2.22 -20--80°C, purified recombinant His-tagged enzyme, 70-80% activity reamining after 70 days Thermus thermophilus
2.4.2.22 4°C, purified recombinant His-tagged enzyme, 85% activity remaining after 70 days Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus AMP + diphosphate
-
r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus DSM 579 AMP + diphosphate
-
r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ATCC 27634 AMP + diphosphate
-
r
2.4.2.7 additional information substrate specificity, overview. TtAPRT2 can use adenine as acceptor for NMP synthesis. But neither guanine nor hypoxanthine or xanthine are recognized as substrates by TtAPRT2 Thermus thermophilus ?
-
-
2.4.2.7 additional information substrate specificity, overview. TtAPRT2 can use adenine as acceptor for NMP synthesis. But neither guanine nor hypoxanthine or xanthine are recognized as substrates by TtAPRT2 Thermus thermophilus DSM 579 ?
-
-
2.4.2.7 additional information substrate specificity, overview. TtAPRT2 can use adenine as acceptor for NMP synthesis. But neither guanine nor hypoxanthine or xanthine are recognized as substrates by TtAPRT2 Thermus thermophilus ATCC 27634 ?
-
-
2.4.2.22 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus GMP + diphosphate
-
r
2.4.2.22 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus DSM 579 GMP + diphosphate
-
r
2.4.2.22 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ATCC 27634 GMP + diphosphate
-
r
2.4.2.22 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate reaction of EC 2.4.2.8 Thermus thermophilus IMP + diphosphate
-
r
2.4.2.22 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate reaction of EC 2.4.2.8 Thermus thermophilus DSM 579 IMP + diphosphate
-
r
2.4.2.22 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate reaction of EC 2.4.2.8 Thermus thermophilus ATCC 27634 IMP + diphosphate
-
r
2.4.2.22 additional information substrate specificity, overview. TtXPRT is confirmed as a xanthine phosphoribosyltransferase. Furthermore, TtXPRT can also slightly recognize hypoxanthine and guanine as substrates, but the activity is low. No activity with adenine Thermus thermophilus ?
-
-
2.4.2.22 additional information substrate specificity, overview. TtXPRT is confirmed as a xanthine phosphoribosyltransferase. Furthermore, TtXPRT can also slightly recognize hypoxanthine and guanine as substrates, but the activity is low. No activity with adenine Thermus thermophilus DSM 579 ?
-
-
2.4.2.22 additional information substrate specificity, overview. TtXPRT is confirmed as a xanthine phosphoribosyltransferase. Furthermore, TtXPRT can also slightly recognize hypoxanthine and guanine as substrates, but the activity is low. No activity with adenine Thermus thermophilus ATCC 27634 ?
-
-
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus XMP + diphosphate
-
r
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus DSM 579 XMP + diphosphate
-
r
2.4.2.22 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ATCC 27634 XMP + diphosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.2.7 homodimer 2 * 19150, about, sequence calculation, 2 * 21000, His-tagged recombinant enzyme TtAPRT2, SDS-PAGE Thermus thermophilus
2.4.2.22 homotetramer 4 * 17100, calculated from amino acid sequence Thermus thermophilus
2.4.2.22 homotetramer 4 * 19020, about, sequence calculation, 4 * 19000, His-tagged recombinant enzyme TtXPRT, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 APRT2
-
Thermus thermophilus
2.4.2.7 TtAPRT2
-
Thermus thermophilus
2.4.2.7 TTHA1614 locus name Thermus thermophilus
2.4.2.22 ttha0535
-
Thermus thermophilus
2.4.2.22 TtXPRT
-
Thermus thermophilus
2.4.2.22 xanthine phosphoribosyltransferase
-
Thermus thermophilus
2.4.2.22 XPRT
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 70
-
recombinant enzyme Thermus thermophilus
2.4.2.22 70
-
recombinant enzyme Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.7 40 90 and above, activity range, profile overview. Recombinant enzyme displays a strong temperature dependence with over 75% of relative activity at 70-90°C Thermus thermophilus
2.4.2.22 30 90 and above, activity range, profile overview. Recombinant enzyme displays a strong temperature dependence with over 75% of relative activity at 60-90°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 8.5
-
recombinant enzyme Thermus thermophilus
2.4.2.22 6
-
recombinant enzyme Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.7 5 10 activity range, recombinant enzyme, highest activity at pH 8.0-10.0, profile overview Thermus thermophilus
2.4.2.22 4 8.5 activity range, recombinant enzyme, highest activity at pH 5.0-6.0, profile overview Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.4.2.7 physiological function purine phosphoribosyltransferases, purine PRTs, are essential enzymes in the purine salvage pathway of living organisms. They are involved in the formation of C-N glycosidic bonds in purine nucleosides-5'-monophosphate (NMPs) through the transfer of the 5-phosphoribosyl group from 5-phospho-alpha-D-ribosyl-1-diphosphate (PRPP) to purine nucleobases in the presence of Mg2+ Thermus thermophilus
2.4.2.22 evolution conserved XPRT PRPP motif sequence comparisons Thermus thermophilus
2.4.2.22 physiological function purine phosphoribosyltransferases, purine PRTs, are essential enzymes in the purine salvage pathway of living organisms. They are involved in the formation of C-N glycosidic bonds in purine nucleosides-5'-monophosphate (NMPs) through the transfer of the 5-phosphoribosyl group from 5-phospho-alpha-D-ribosyl-1-diphosphate (PRPP) to purine nucleobases in the presence of Mg2+ Thermus thermophilus