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Literature summary extracted from

  • Bezsudnova, E.Y.; Stekhanova, T.N.; Popinako, A.V.; Rakitina, T.V.; Nikolaeva, A.Y.; Boyko, K.M.; Popov, V.O.
    Diaminopelargonic acid transaminase from Psychrobacter cryohalolentis is active towards (S)-(-)-1-phenylethylamine, aldehydes and alpha-diketones (2018), Appl. Microbiol. Biotechnol., 102, 9621-9633 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.62 gene Pcryo-0361, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21Star Psychrobacter cryohalolentis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.62 purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 11 mg/ml protein in 50 mM Tris, 100 mM NaCl, and 0.02 mM PLP, pH 7.9, with precipitant solution containing 0.1 M sodium citrate, pH 6.1, 0.2 M potassium sodium tartrate, and 1.8 M ammonium sulfate. A glass capillary containing 0.008 ml of protein solution is mounted in the gel tube with 1% agarose presoaked in 1 ml of the precipitant solution, method optimization, 1 month at 20°C. X-ray diffraction structure determination and analysis at 1.6 A resolution Psychrobacter cryohalolentis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 additional information
-
 additional information kinetic analysis of the half-reaction with different substrates Psychrobacter cryohalolentis
2.6.1.62 0.22
-
 2,3-Butanedione pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis
2.6.1.62 1.4
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with isobutyraldehyde Psychrobacter cryohalolentis
2.6.1.62 3.87
-
 Isobutyraldehyde pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis
2.6.1.62 8.2
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with 2,3-butanedione Psychrobacter cryohalolentis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Psychrobacter cryohalolentis
-
 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Psychrobacter cryohalolentis DSM 17306
-
 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Psychrobacter cryohalolentis K5
-
 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Psychrobacter cryohalolentis VKM B-2378
-
 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Psychrobacter cryohalolentis ATCC BAA-1226
-
 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Psychrobacter cryohalolentis Q1QDV8
-
-
2.6.1.62 Psychrobacter cryohalolentis ATCC BAA-1226 Q1QDV8
-
-
2.6.1.62 Psychrobacter cryohalolentis DSM 17306 Q1QDV8
-
-
2.6.1.62 Psychrobacter cryohalolentis K5 Q1QDV8
-
-
2.6.1.62 Psychrobacter cryohalolentis VKM B-2378 Q1QDV8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 2,3-butanedione + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 2,3-butanedione + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis DSM 17306 ?
-
 ?
2.6.1.62 2,3-butanedione + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis K5 ?
-
 ?
2.6.1.62 2,3-butanedione + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis VKM B-2378 ?
-
 ?
2.6.1.62 2,3-butanedione + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis ATCC BAA-1226 ?
-
 ?
2.6.1.62 2,3-dihydroxypropanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 2,3-dihydroxypropanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis DSM 17306 ?
-
 ?
2.6.1.62 2,3-dihydroxypropanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis K5 ?
-
 ?
2.6.1.62 2,3-dihydroxypropanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis VKM B-2378 ?
-
 ?
2.6.1.62 2,3-dihydroxypropanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ATCC BAA-1226 ?
-
 ?
2.6.1.62 2,3-hexanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 2,3-hexanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis DSM 17306 ?
-
 ?
2.6.1.62 2,3-hexanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis K5 ?
-
 ?
2.6.1.62 2,3-hexanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis VKM B-2378 ?
-
 ?
2.6.1.62 2,3-hexanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ATCC BAA-1226 ?
-
 ?
2.6.1.62 2,3-pentanedione + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 2-oxo-2-phenylacetaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 benzaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis propiophenone + ?
-
 ?
2.6.1.62 glyoxylate + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 hexanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 isobutyraldehyde + (S)-(-)-1-phenylethylamine molecular docking study Psychrobacter cryohalolentis acetophenone + ?
-
 ?
2.6.1.62 isobutyraldehyde + (S)-(-)-1-phenylpropylamine
-
 Psychrobacter cryohalolentis acetophenone + ?
-
 ?
2.6.1.62 additional information 7,8-diaminopelargonic acid transaminase (synthase), previously considered to be highly specific, is able to convert (S)-(-)-1-phenylethylamine and a number of aldehydes and diketones. Only a weak activity towards pyruvate is detected. The enzyme shows (S)-amine transaminase activity. The Pcryo361 affinity towards (S)-(-)-1-phenylethylamine arises from the recognition of the hydrophobic parts of the specific substrates, S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid, and from the flexibility of the active site. Analysis of the half-transamination reaction measuring a decrease in aldimine concentration in the presence of different concentrations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, L-lysine, and (S)-(-)-1,2-diaminopropane, substrate specificity of enzyme Pcryo361 in the half and the overall reactions, overview. No activity towards 2,4-pentanedione, 2-oxobutyrate, or acetone as amine acceptor substrates, poor activity with pyruvate, no activity with (R)-(-)-1-phenylethylamine as amine donor substrate Psychrobacter cryohalolentis ?
-
-
2.6.1.62 additional information 7,8-diaminopelargonic acid transaminase (synthase), previously considered to be highly specific, is able to convert (S)-(-)-1-phenylethylamine and a number of aldehydes and diketones. Only a weak activity towards pyruvate is detected. The enzyme shows (S)-amine transaminase activity. The Pcryo361 affinity towards (S)-(-)-1-phenylethylamine arises from the recognition of the hydrophobic parts of the specific substrates, S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid, and from the flexibility of the active site. Analysis of the half-transamination reaction measuring a decrease in aldimine concentration in the presence of different concentrations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, L-lysine, and (S)-(-)-1,2-diaminopropane, substrate specificity of enzyme Pcryo361 in the half and the overall reactions, overview. No activity towards 2,4-pentanedione, 2-oxobutyrate, or acetone as amine acceptor substrates, poor activity with pyruvate, no activity with (R)-(-)-1-phenylethylamine as amine donor substrate Psychrobacter cryohalolentis DSM 17306 ?
-
-
2.6.1.62 additional information 7,8-diaminopelargonic acid transaminase (synthase), previously considered to be highly specific, is able to convert (S)-(-)-1-phenylethylamine and a number of aldehydes and diketones. Only a weak activity towards pyruvate is detected. The enzyme shows (S)-amine transaminase activity. The Pcryo361 affinity towards (S)-(-)-1-phenylethylamine arises from the recognition of the hydrophobic parts of the specific substrates, S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid, and from the flexibility of the active site. Analysis of the half-transamination reaction measuring a decrease in aldimine concentration in the presence of different concentrations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, L-lysine, and (S)-(-)-1,2-diaminopropane, substrate specificity of enzyme Pcryo361 in the half and the overall reactions, overview. No activity towards 2,4-pentanedione, 2-oxobutyrate, or acetone as amine acceptor substrates, poor activity with pyruvate, no activity with (R)-(-)-1-phenylethylamine as amine donor substrate Psychrobacter cryohalolentis K5 ?
-
-
2.6.1.62 additional information 7,8-diaminopelargonic acid transaminase (synthase), previously considered to be highly specific, is able to convert (S)-(-)-1-phenylethylamine and a number of aldehydes and diketones. Only a weak activity towards pyruvate is detected. The enzyme shows (S)-amine transaminase activity. The Pcryo361 affinity towards (S)-(-)-1-phenylethylamine arises from the recognition of the hydrophobic parts of the specific substrates, S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid, and from the flexibility of the active site. Analysis of the half-transamination reaction measuring a decrease in aldimine concentration in the presence of different concentrations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, L-lysine, and (S)-(-)-1,2-diaminopropane, substrate specificity of enzyme Pcryo361 in the half and the overall reactions, overview. No activity towards 2,4-pentanedione, 2-oxobutyrate, or acetone as amine acceptor substrates, poor activity with pyruvate, no activity with (R)-(-)-1-phenylethylamine as amine donor substrate Psychrobacter cryohalolentis VKM B-2378 ?
-
-
2.6.1.62 additional information 7,8-diaminopelargonic acid transaminase (synthase), previously considered to be highly specific, is able to convert (S)-(-)-1-phenylethylamine and a number of aldehydes and diketones. Only a weak activity towards pyruvate is detected. The enzyme shows (S)-amine transaminase activity. The Pcryo361 affinity towards (S)-(-)-1-phenylethylamine arises from the recognition of the hydrophobic parts of the specific substrates, S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid, and from the flexibility of the active site. Analysis of the half-transamination reaction measuring a decrease in aldimine concentration in the presence of different concentrations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, L-lysine, and (S)-(-)-1,2-diaminopropane, substrate specificity of enzyme Pcryo361 in the half and the overall reactions, overview. No activity towards 2,4-pentanedione, 2-oxobutyrate, or acetone as amine acceptor substrates, poor activity with pyruvate, no activity with (R)-(-)-1-phenylethylamine as amine donor substrate Psychrobacter cryohalolentis ATCC BAA-1226 ?
-
-
2.6.1.62 propanaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 pyruvaldehyde + (S)-(-)-1-phenylethylamine
-
 Psychrobacter cryohalolentis ?
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Psychrobacter cryohalolentis S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Psychrobacter cryohalolentis DSM 17306 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Psychrobacter cryohalolentis K5 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Psychrobacter cryohalolentis VKM B-2378 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Psychrobacter cryohalolentis ATCC BAA-1226 S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.62 7,8-diaminopelargonic acid synthase
-
 Psychrobacter cryohalolentis
2.6.1.62 7,8-diaminopelargonic acid transaminase
-
 Psychrobacter cryohalolentis
2.6.1.62 diaminopelargonic acid transaminase
-
 Psychrobacter cryohalolentis
2.6.1.62 More cf. EC 2.6.1, (S)-specific amine transaminase ((S)-ATA) Psychrobacter cryohalolentis
2.6.1.62 Pcryo_0361
-
 Psychrobacter cryohalolentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.62 35 42
-
 Psychrobacter cryohalolentis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.62 0.026
-
 2,3-Butanedione pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis
2.6.1.62 0.045
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with 2,3-butanedione Psychrobacter cryohalolentis
2.6.1.62 0.16
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with isobutyraldehyde Psychrobacter cryohalolentis
2.6.1.62 0.25
-
 Isobutyraldehyde pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.62 9 11
-
 Psychrobacter cryohalolentis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.62 pyridoxal 5'-phosphate PLP Psychrobacter cryohalolentis

General Information

EC Number General Information Comment Organism
2.6.1.62 evolution the enzyme belongs to class III of the superfamily of aminotransferases (transaminase, TA) Psychrobacter cryohalolentis
2.6.1.62 additional information the conversion of amines is a promiscuous activity of many transaminases of class III and is independent from their natural function. Molecular docking calculations of S-adenosyl-L-methionine, (S)-(-)-1-phenylethylamine, and its quinonoid intermediates in the active site of the enzyme Psychrobacter cryohalolentis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.62 0.0055
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with 2,3-butanedione Psychrobacter cryohalolentis
2.6.1.62 0.065
-
 Isobutyraldehyde pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis
2.6.1.62 0.11
-
 (S)-(-)-1-phenylethylamine pH 8.0, 35°C, with isobutyraldehyde Psychrobacter cryohalolentis
2.6.1.62 0.12
-
 2,3-Butanedione pH 8.0, 35°C, with (S)-(-)-1-phenylethylamine Psychrobacter cryohalolentis