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Literature summary extracted from

  • Chaudhary, S.K.; Jeyakanthan, J.; Sekar, K.
    Structural and functional roles of dynamically correlated residues in thymidylate kinase (2018), Acta Crystallogr. Sect. D, 74, 341-354 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.9 high-resolution X-ray crystal structures of thymidylate kinase in apo and ligand-bound states, structures of binary and ternary complexes of thymidylate kinase with its natural substrates ATP and ATP-TMP, respectively Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.9 D90L high similarity to the wild-type. Less than 10% of wild-type activity Thermus thermophilus
2.7.4.9 K16M high similarity to the wild-type. Less than 10% of wild-type activity Thermus thermophilus
2.7.4.9 T18V equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure. Increase in thermal stability by 3.4°C Thermus thermophilus
2.7.4.9 V158T equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure, thermal stability similar to that of wild-type enzyme Thermus thermophilus
2.7.4.9 Y162F equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure Thermus thermophilus
2.7.4.9 Y162F significant decrease in thermal stability of 10 °C as compared with wild-type enzyme Thermus thermophilus
2.7.4.9 Y92H equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure, significant decrease in thermal stability of 8.8 °C as compared with wild-type enzyme Thermus thermophilus
2.7.4.9 Y99F high similarity to the wild-type, activity is about 60% of the wild-type activity Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Mg2+ required Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.9 ATP + dTMP Thermus thermophilus
-
 ADP + dTDP
-
 ?
2.7.4.9 ATP + dTMP Thermus thermophilus ATCC 27634
-
 ADP + dTDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Thermus thermophilus Q5SHX3
-
-
2.7.4.9 Thermus thermophilus ATCC 27634 Q5SHX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dTMP
-
 Thermus thermophilus ADP + dTDP
-
 ?
2.7.4.9 ATP + dTMP
-
 Thermus thermophilus ATCC 27634 ADP + dTDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 89.2
-
 Tm-value of wild-type enzyme Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.7.4.9 physiological function important enzyme in DNA synthesis Thermus thermophilus