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Literature summary extracted from

  • Rastogi, S.; Singh, A.K.; Chandra, G.; Kushwaha, P.; Pant, G.; Singh, K.; Mitra, K.; Sashidhara, K.V.; Krishnan, M.Y.
    The diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations (2017), Tuberculosis, 104, 8-19 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene Rv3371, specific to pathogenic mycobacteria, leads to modifications in colony morphotype, bacterial architecture, cell surface properties, and to elevated triacylglycerol (TAG) levels when expressed in Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 cell membrane mainly Mycobacterium tuberculosis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis H37Rv
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Mycobacterium tuberculosis ATCC 25618
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Mycobacterium tuberculosis P9WKA9
-
-
2.3.1.20 Mycobacterium tuberculosis ATCC 25618 P9WKA9
-
-
2.3.1.20 Mycobacterium tuberculosis H37Rv P9WKA9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis H37Rv CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mycobacterium tuberculosis ATCC 25618 CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT
-
 Mycobacterium tuberculosis
2.3.1.20 diacylglycerol acyltransferase
-
 Mycobacterium tuberculosis
2.3.1.20 Rv3371
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
2.3.1.20 Mycobacterium tuberculosis the Rv3371 promoter is minimally active during exponential growth in vitro, but is upregulated under stationary phase, hypoxia, nutrient starvation, nitrosative stress, low iron, in IFN-gamma activated macrophages and infected mice. The low iron-induced expression of Rv3371 is likely due to the de-repression by Rv1404, which is probably activated by ideR up

General Information

EC Number General Information Comment Organism
2.3.1.20 malfunction a Rv3371 deletion mutant of Mtb shows impaired non-replicating persistence in vitro and altered sensitivity to anti-mycobacterial drugs. In low iron medium, the Rv3371 deletion mutant shows reduced formation of TAG containing extracellular vesicles Mycobacterium tuberculosis
2.3.1.20 physiological function the diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations during persistence. It is involved in the biosynthesis of triacylglycerol (TAG). TAG is important to mycobacteria both as cell envelope component and energy reservoir Mycobacterium tuberculosis