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Literature summary extracted from

  • Li, Y.M.; Milne, J.C.; Madison, L.L.; Kolter, R.; Walsh, C.T.
    From peptide precursors to oxazole and thiazole-containing peptide antibiotics microcin B17 synthase (1996), Science, 274, 1188-1193 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.16 a [protein]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline + O2 Escherichia coli
-
 a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-thiazole + H2O2
-
 ?
1.3.3.16 a [protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-2-oxazoline + O2 Escherichia coli
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 a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-oxazole + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.16 Escherichia coli P23184 and P23185 and P23186 P23184 i.e. subunit McbB, P23185 i.e. subunit McBC, P23186 i.e. subunit McbD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.16 a [protein]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline + O2
-
 Escherichia coli a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-thiazole + H2O2
-
 ?
1.3.3.16 a [protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-2-oxazoline + O2
-
 Escherichia coli a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-oxazole + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.16 ? x * 33000, subunit McbB, x * 31000, subunit McbC, x * 45000, subunit McbD, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.3.3.16 McbB
-
 Escherichia coli
1.3.3.16 McbC
-
 Escherichia coli
1.3.3.16 McbD
-
 Escherichia coli
1.3.3.16 microcin B17 synthase
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.3.3.16 physiological function enzyme is part of microcin B17 synthase. The complex three proteins, McbB, McbC, and McbD, that convert 14 residues into the eight mono- and bisheterocyclic moieties in vitro that confer antibiotic activity on mature microcin B17 via a three-step sequence of cyclization, net dehydration, and subsequent two-electron dehydrogenation Escherichia coli