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Literature summary extracted from

  • Pathak, D.; Bhat, A.; Sapehia, V.; Rai, J.; Rao, A.
    Biochemical evidence for relaxed substrate specificity of Nalpha-acetyltransferase (Rv3420c/rimI) of Mycobacterium tuberculosis (2016), Sci. Rep., 6, 28892 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.255 gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli Mycobacterium tuberculosis
2.3.1.256 gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli Mycobacterium tuberculosis
2.3.1.258 gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.255 Mycobacterium tuberculosis I6YG32
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-
2.3.1.255 Mycobacterium tuberculosis ATCC 25618 I6YG32
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-
2.3.1.255 Mycobacterium tuberculosis H37Rv I6YG32
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-
2.3.1.256 Mycobacterium tuberculosis I6YG32
-
-
2.3.1.256 Mycobacterium tuberculosis ATCC 25618 I6YG32
-
-
2.3.1.256 Mycobacterium tuberculosis H37Rv I6YG32
-
-
2.3.1.258 Mycobacterium tuberculosis I6YG32
-
-
2.3.1.258 Mycobacterium tuberculosis ATCC 25618 I6YG32
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-
2.3.1.258 Mycobacterium tuberculosis H37Rv I6YG32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.255 recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity and gel filtration Mycobacterium tuberculosis
2.3.1.258 recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.255 600
-
 about, purified recombinant RimI, NatA substrate N-terminal L-alanyl-[RYFRR], pH 8.0, 25°C Mycobacterium tuberculosis
2.3.1.256 1000
-
 about, purified recombinant RimI, NatC substrate N-terminal L-methionyl-L-leucyl-[RYFRR], pH 8.0, 25°C Mycobacterium tuberculosis
2.3.1.258 1300
-
 about, purified recombinant RimI, NatE substrate N-terminal L-methionyl-[ARYFRR], pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
-
 ir
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
-
 ir
2.3.1.255 additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis ?
-
-
2.3.1.255 additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis H37Rv ?
-
-
2.3.1.255 additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis ATCC 25618 ?
-
-
2.3.1.256 acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[RYFRR] DP11 peptide (MARYFRR) is a substrate of NatC, a synthetic peptide Mycobacterium tuberculosis N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[RYFRR] + CoA
-
 ir
2.3.1.256 additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. The NatB substrate peptide MERYFRR (DP10) is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed gainst peptide representing N-terminus of GroES. RimIMtb acetylates DP11 (NatC substrate) about 1.7fold better than DPC (NatA substrate). RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins Mycobacterium tuberculosis ?
-
-
2.3.1.258 acetyl-CoA + N-terminal L-methionyl-[ARYFRR] DP9 peptide (MARYFRR) is a substrate of NatE, a synthetic peptide Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[ARYFRR]
-
 ir
2.3.1.258 additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed gainst peptide representing N-terminus of GroES. RimIMtb acetylates DP9 (NatE substrate) 2.1fold better than DPC (NatA substrate). RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins Mycobacterium tuberculosis ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.255 Nalpha-acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.255 NatA
-
 Mycobacterium tuberculosis
2.3.1.255 RimI
-
 Mycobacterium tuberculosis
2.3.1.255 RimI acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.255 Rv3420c
-
 Mycobacterium tuberculosis
2.3.1.256 Nalpha-acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.256 NatC
-
 Mycobacterium tuberculosis
2.3.1.256 RimI
-
 Mycobacterium tuberculosis
2.3.1.256 RimI acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.256 Rv3420c
-
 Mycobacterium tuberculosis
2.3.1.258 Nalpha-acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.258 NatE
-
 Mycobacterium tuberculosis
2.3.1.258 RimI
-
 Mycobacterium tuberculosis
2.3.1.258 RimI acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.258 Rv3420c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.255 25
-
 assay at Mycobacterium tuberculosis
2.3.1.256 25
-
 assay at Mycobacterium tuberculosis
2.3.1.258 25
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.255 8
-
 assay at Mycobacterium tuberculosis
2.3.1.256 8
-
 assay at Mycobacterium tuberculosis
2.3.1.258 8
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.255 acetyl-CoA
-
 Mycobacterium tuberculosis
2.3.1.256 acetyl-CoA
-
 Mycobacterium tuberculosis
2.3.1.258 acetyl-CoA
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.255 physiological function Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs) Mycobacterium tuberculosis
2.3.1.256 physiological function Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs). RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed gainst peptide representing N-terminus of GroES Mycobacterium tuberculosis
2.3.1.258 physiological function Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs) Mycobacterium tuberculosis