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Literature summary extracted from

  • Zhang, H.; Sun, X.; Li, W.; Li, T.; Li, S.; Kitaoka, M.
    Expression and characterization of recombinant sucrose phosphorylase (2018), Protein J., 37, 93-100 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.7 industry the enzyme is widely used in the food, cosmetics, and pharmaceutical industries Bifidobacterium longum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.7 expression in Escherichia coli BL21. Expression conditions are optimized. The highest expression efficiency is obtained at an initial cell density of OD600 0.5, with 0.05 mM isopropyl-beta-D-thiogalactoside, followed by shaking at 180 rpm and incubation at 30°C for 15 h Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 56510
-
 SDS-PAGE, calculated from sequence Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Bifidobacterium longum A5A8M5
-
-
2.4.1.7 Bifidobacterium longum JCM1217 A5A8M5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7 6 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 122.1
-
 pH and temperature not specified in the publication Bifidobacterium longum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 SPase
-
 Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 45
-
-
 Bifidobacterium longum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.7 20 90 20°C: about 55% of maximal activity, 90°C: about 80% of maximal activity Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.7 30 55 1 h, the enzyme retains more than 80% relative activity at 30-55°C Bifidobacterium longum
2.4.1.7 60 90 1 h, the enzyme retains more than 70% relative activity at 60-90°C Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.5
-
-
 Bifidobacterium longum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5.5 7 pH 5.5: about 45% of maximal activity, pH 7.0: about 55% of maximal activity Bifidobacterium longum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.7 5
-
 45°C, 1 h, 80% loss of activity Bifidobacterium longum
2.4.1.7 6 6.5 45°C, 1 h, stable Bifidobacterium longum
2.4.1.7 7
-
 45°C, 1 h, 25% loss of activity Bifidobacterium longum
2.4.1.7 7.5
-
 45°C, 1 h, 60% loss of activity Bifidobacterium longum