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Literature summary extracted from

  • Yao, D.; Fan, J.; Han, R.; Xiao, J.; Li, Q.; Xu, G.; Dong, J.; Ni, Y.
    Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones (2020), Protein Expr. Purif., 169, 105571 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.7 industry the enzyme has a wide range of application in food, cosmetics, and pharmaceutical industries because of its broad substrate specificity Thermoanaerobacterium thermosaccharolyticum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.7 expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized Thermoanaerobacterium thermosaccharolyticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 55.6
-
sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 56000
-
SDS-PAGE Thermoanaerobacterium thermosaccharolyticum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Thermoanaerobacterium thermosaccharolyticum WP_094046414.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7
-
Thermoanaerobacterium thermosaccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 sucrose + hydroquinone
-
Thermoanaerobacterium thermosaccharolyticum D-fructose + alpha-arbutin
-
?
2.4.1.7 sucrose + phosphate
-
Thermoanaerobacterium thermosaccharolyticum D-fructose + alpha-D-glucose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.7 ? x * 56000, SDS-PAGE Thermoanaerobacterium thermosaccharolyticum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 SPase
-
Thermoanaerobacterium thermosaccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 60
-
-
Thermoanaerobacterium thermosaccharolyticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.7 40 70 40°C: about 45% of maximal activity, 70°C: about 85% of maximal activity Thermoanaerobacterium thermosaccharolyticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.7 70
-
half-life: 19 min Thermoanaerobacterium thermosaccharolyticum
2.4.1.7 73
-
melting temperature Thermoanaerobacterium thermosaccharolyticum
2.4.1.7 80
-
half-life: 2.3 min Thermoanaerobacterium thermosaccharolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.7 100
-
sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.5
-
-
Thermoanaerobacterium thermosaccharolyticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5.5 8 pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity Thermoanaerobacterium thermosaccharolyticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.7 6 7.5 1 h, the enzyme retains more than 50% of its activity Thermoanaerobacterium thermosaccharolyticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.7 1.8
-
sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum