EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.7 | industry | the enzyme has a wide range of application in food, cosmetics, and pharmaceutical industries because of its broad substrate specificity | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.7 | expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized | Thermoanaerobacterium thermosaccharolyticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.7 | 55.6 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 56000 | - |
SDS-PAGE | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.7 | Thermoanaerobacterium thermosaccharolyticum | WP_094046414.1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.7 | - |
Thermoanaerobacterium thermosaccharolyticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.7 | sucrose + hydroquinone | - |
Thermoanaerobacterium thermosaccharolyticum | D-fructose + alpha-arbutin | - |
? | |
2.4.1.7 | sucrose + phosphate | - |
Thermoanaerobacterium thermosaccharolyticum | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.7 | ? | x * 56000, SDS-PAGE | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.7 | SPase | - |
Thermoanaerobacterium thermosaccharolyticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 60 | - |
- |
Thermoanaerobacterium thermosaccharolyticum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 40 | 70 | 40°C: about 45% of maximal activity, 70°C: about 85% of maximal activity | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 70 | - |
half-life: 19 min | Thermoanaerobacterium thermosaccharolyticum |
2.4.1.7 | 73 | - |
melting temperature | Thermoanaerobacterium thermosaccharolyticum |
2.4.1.7 | 80 | - |
half-life: 2.3 min | Thermoanaerobacterium thermosaccharolyticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.7 | 100 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 6.5 | - |
- |
Thermoanaerobacterium thermosaccharolyticum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity | Thermoanaerobacterium thermosaccharolyticum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 6 | 7.5 | 1 h, the enzyme retains more than 50% of its activity | Thermoanaerobacterium thermosaccharolyticum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.7 | 1.8 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum |