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Literature summary extracted from

  • Sodolescu, A.; Dian, C.; Terradot, L.; Bouzhir-Sima, L.; Lestini, R.; Myllykallio, H.; Skouloubris, S.; Liebl, U.
    Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori (2018), PLoS ONE, 13, e0208850 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli SG 13009 cells Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M sodium acetate Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Helicobacter pylori
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Helicobacter pylori 26695
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Helicobacter pylori
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine Helicobacter pylori 26695
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Helicobacter pylori P56089
-
-
2.1.2.1 Helicobacter pylori 26695 P56089
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 Ni-TED column chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Helicobacter pylori tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Helicobacter pylori 26695 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Helicobacter pylori 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Helicobacter pylori 26695 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homodimer 2 * 45000, SDS-PAGE Helicobacter pylori
2.1.2.1 homodimer 2 * 46776, calculated from amino acid sequence Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 GlyA
-
 Helicobacter pylori
2.1.2.1 serine hydroxymethyltransferase
-
 Helicobacter pylori
2.1.2.1 SHMT
-
 Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Helicobacter pylori