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Literature summary extracted from

  • Subramanian, P.; Krishnamoorthy, R.; Chanratana, M.; Kim, K.; Sa, T.
    Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in psychrotolerant bacteria modulates ethylene metabolism and cold induced genes in tomato under chilling stress (2015), Plant Physiol. Biochem., 89, 18-23 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.7 gene acdS, functional recombinant expression of the exogenous 1-aminocyclopropane-1-carboxylate deaminase gene from Pseudomonas putida in the psychrotolerant bacteria, acdS negative Flavobacterium sp. strain OR306 and Pseudomonas frederiksbergensis strain OS211 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
3.5.99.7 additional information gene acdS expressing mutant strains are generated from ACCD negative psychrotolerant bacterial strains Flavobacterium sp. OR306 and Pseudomonas frederiksbergensis OS211, isolated from agricultural soil during late winter. After transformation with plasmid pRKACC-acdS, both the strains are able to exhibit ACCD activity in vitro. On exposing tomato plants inoculated with both acdS expressing and wild-type bacteria to one week of chilling treatment at 12/10°C, stress ethylene, ACC accumulation and ACO activity, which are markers of ethylene stress, are significantly reduced in plants inoculated with the acdS gene transformed mutants. In case of plants inoculated with strain OS211-acdS, ethylene emission, ACC accumulation and ACO activity are significantly reduced by 52%, 75.9%, and 23.2%, respectively, compared to uninoculated control plants. Expression analysis of cold-induced LeCBF1 and LeCBF3 genes shows that these genes in the plants are significantly induced by the acdS transformed mutant bacteria in addition to observed reduced expression of ethylene-responsive transcription factor 13 (ETF-13) and ACO genes Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.7 Pseudomonas putida Q5PWZ8
-
-
3.5.99.7 Pseudomonas putida UW4 Q5PWZ8
-
-

Synonyms

EC Number Synonyms Comment Organism
3.5.99.7 ACC deaminase
-
 Pseudomonas putida
3.5.99.7 AcdS
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.99.7 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.7 7.2
-
 assay at Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.5.99.7 physiological function recombinant expression of the exogenous 1-aminocyclopropane-1-carboxylate deaminase gene from Pseudomonas putida in psychrotolerant bacteria, i.e. acdS-deficient Flavobacterium sp. strain OR306 and Pseudomonas frederiksbergensis strain OS211, modulates ethylene metabolism and cold-induced genes in tomato under chilling stress. Physiologically evolved stress ethylene and its transcription factors play a role in regulation of cold-induced genes, e.g. LeCBF1 and LeCBF3 Pseudomonas putida