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Literature summary extracted from

  • Tanaka, T.; Hosaka, K.; Numa, S.
    Long-chain acyl-CoA synthetase from rat liver (1981), Methods Enzymol., 71, 334-341.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.3 stable to 24 h dialysis at 0EC, against 50 mM potassium phosphate buffer, pH 7.4, containing 2 mM Triton X-100, 2 mM DTT, 1 mM EDTA Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.026 0.028 CoA
-
 Rattus norvegicus
6.2.1.3 0.23 0.29 dephospho-CoA
-
 Rattus norvegicus
6.2.1.3 0.41 0.43 4'-phosphopantetheine
-
 Rattus norvegicus
6.2.1.3 1.1 1.2 pantetheine
-
 Rattus norvegicus
6.2.1.3 2.3 2.4 ATP
-
 Rattus norvegicus
6.2.1.3 12.5 13.3 dATP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 microsome
-
 Rattus norvegicus
-
-
6.2.1.3 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.3 76000
-
 x * 76000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 26.2
-
 mitochondrial enzyme Rattus norvegicus
6.2.1.3 28.7
-
 microsomal enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.2.1.3 -70°C, stable for at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + fatty acid + 4'-phosphopantetheine
-
 Rattus norvegicus AMP + diphosphate + acyl-4'-phosphopantetheine
-
 ?
6.2.1.3 ATP + fatty acid + dephospho-CoA
-
 Rattus norvegicus AMP + diphosphate + acyl-dephospho-CoA
-
 ?
6.2.1.3 ATP + fatty acid + pantetheine
-
 Rattus norvegicus AMP + diphosphate + acyl-pantetheine
-
 ?
6.2.1.3 dATP + fatty acid + CoA
-
 Rattus norvegicus dAMP + diphosphate + acyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 ? x * 76000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.3 7.4 9.1
-
 Rattus norvegicus