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Literature summary extracted from

  • Yang, Q.; Deng, W.; Wang, S.; Liu, H.; Li, X.; Zheng, M.
    Effects of resistance mutations of Pro197, Asp376 and Trp574 on the characteristics of acetohydroxyacid synthase (AHAS) isozymes (2018), Pest Manag. Sci., 74, 1870-1879 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 D376E the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
2.2.1.6 P197H the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
2.2.1.6 P197L the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
2.2.1.6 P197T the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia
2.2.1.6 W574L the resistance mutation causes more than 200fold resistance to tribenuron-methyl and also greatly reduces the enzyme sensitivity to tribenuron-methyl and increases enzyme binding affinity for the substrate pyruvate Descurainia sophia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 L-isoleucine
-
 Descurainia sophia
2.2.1.6 L-leucine
-
 Descurainia sophia
2.2.1.6 L-valine
-
 Descurainia sophia
2.2.1.6 tribenuron-methyl
-
 Descurainia sophia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 1.09
-
 pyruvate mutant enzyme W574L, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.19
-
 pyruvate mutant enzyme P197T, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.37
-
 pyruvate mutant enzyme P197L, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.38
-
 pyruvate mutant enzyme P197H, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.46
-
 pyruvate mutant enzyme P197S, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.5 2 pyruvate mutant enzyme D376E, at pH 7.5 and 37°C Descurainia sophia
2.2.1.6 1.74
-
 pyruvate wild type enzyme, at pH 7.5 and 37°C Descurainia sophia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ 0.5 mM used in assay conditions Descurainia sophia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Descurainia sophia
-
 2-acetolactate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Descurainia sophia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 ammonium sulfate precipitation and Sephadex G-25 gel filtration Descurainia sophia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Descurainia sophia 2-acetolactate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Descurainia sophia
2.2.1.6 acetolactate synthase
-
 Descurainia sophia
2.2.1.6 AHAS
-
 Descurainia sophia
2.2.1.6 ALS1
-
 Descurainia sophia

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD
-
 Descurainia sophia
2.2.1.6 thiamine diphosphate
-
 Descurainia sophia