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Literature summary extracted from

  • Wu, X.; Hou, Z.; Huang, C.; Chen, Q.; Gao, W.; Zhang, J.
    Cloning, purification and characterization of trehalose-6-phosphate synthase from Pleurotus tuoliensis (2018), PeerJ, 6, e5230 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 chondroitin sulfate maximum activity at 0.0002 mg chondroitin sulfate Pleurotus tuoliensis
2.4.1.15 heparin maximum activity at 0.0001 mg heparin Pleurotus tuoliensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 expressed in Escherichia coli BL21(DE3) cells Pleurotus tuoliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 Ca2+ 40.28% residual activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Cd2+ 40.28% residual activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 CDTA 0.08% residual activity at 2.5 mM Pleurotus tuoliensis
2.4.1.15 Citric acid 3.55% residual activity at 2.5 mM Pleurotus tuoliensis
2.4.1.15 EDTA 0.43% residual activity at 2.5 mM Pleurotus tuoliensis
2.4.1.15 EGTA 0.22% residual activity at 2.5 mM Pleurotus tuoliensis
2.4.1.15 Fe3+ 34.53% residual activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Li+ 36.45% residual activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Na+ 21.1% residual activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Sodium citrate 93.98% residual activity at 2.5 mM Pleurotus tuoliensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 Ag+ 222.51% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Co2+ 1922.04% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Cu2+ 124.68% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Fe2+ 161.13% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 K+ 287.73% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Mg2+ most appropriate cation to activate enzyme activity as it increases the activity by 7851fold at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Mn2+ 475.71% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Ni2+ 402.82% activity at 12.5 mM Pleurotus tuoliensis
2.4.1.15 Zn2+ 163.05% activity at 12.5 mM Pleurotus tuoliensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Pleurotus tuoliensis
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Pleurotus tuoliensis CCMSSC 00489
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Pleurotus tuoliensis
-
 previously named Pleurotus eryngii var. tuoliensis
-
2.4.1.15 Pleurotus tuoliensis CCMSSC 00489
-
 previously named Pleurotus eryngii var. tuoliensis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 Ni-NTA agarose column chromatography Pleurotus tuoliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 additional information GDP-alpha-D-glucose and ADP-alpha-D-glucose give little enzyme activity. Glucosamine 6-phosphate and fructose 6-phosphate have almost no enzyme activity, while mannose 6-phosphate exhibits little activity Pleurotus tuoliensis ?
-
-
2.4.1.15 additional information GDP-alpha-D-glucose and ADP-alpha-D-glucose give little enzyme activity. Glucosamine 6-phosphate and fructose 6-phosphate have almost no enzyme activity, while mannose 6-phosphate exhibits little activity Pleurotus tuoliensis CCMSSC 00489 ?
-
-
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Pleurotus tuoliensis UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Pleurotus tuoliensis CCMSSC 00489 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.15 ? x * 60000, SDS-PAGE Pleurotus tuoliensis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 TPS
-
 Pleurotus tuoliensis
2.4.1.15 trehalose-6-phosphate synthase
-
 Pleurotus tuoliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 30
-
-
 Pleurotus tuoliensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.15 20 40 more than 59% activity between 20 and 40°C, the enzyme activity declines abruptly as the temperature climbed to 50°C and keeps constantly around 10% of the maximum until 90°C Pleurotus tuoliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 7
-
-
 Pleurotus tuoliensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.15 7 9 more than 50% activity exists at pH 7.0-9.0 in Tris/HCl buffer Pleurotus tuoliensis

Expression

EC Number Organism Comment Expression
2.4.1.15 Pleurotus tuoliensis enzyme expression increases 24-48 h after heat treatment (37°C) up