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Literature summary extracted from

  • Ochaya, S.; Franzen, O.; Buhwa, D.A.; Foyn, H.; Butler, C.E.; Stove, S.I.; Tyler, K.M.; Arnesen, T.; Matovu, E.; Aslund, L.; Andersson, B.
    Characterization of evolutionarily conserved Trypanosoma cruzi NatC and NatA-N-terminal acetyltransferase complexes (2019), J. Parasitol. Res., 2019, 6594212 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.255 genes TcNaa15 and TcNaa10, DNA and amino acid sequence determination, comparison, and analysis Trypanosoma cruzi
2.3.1.256 genes encoding TcNaa30, TcNaa35, and TcNaa38, DNA and amino acid sequence determination, comparison, and analysis, recombinant expression of GST-tagged subunits Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.255 cytoplasm TcNaa15 and TcNaa10 Trypanosoma cruzi 5737
-
2.3.1.255 additional information TcNaa15 and TcNaa10 both proteins show nuclear and cytoplasmic location. TcNatC and TcNatA proteins physically interact with each other and it is plausible that this interaction takes place in the cytoplasm as suggested by their possible ribosomal cosedimentation. Subcellular localization of subunits, overview Trypanosoma cruzi
-
-
2.3.1.255 nucleus TcNaa15 and TcNaa10 Trypanosoma cruzi 5634
-
2.3.1.256 cytoplasm
-
 Trypanosoma cruzi 5737
-
2.3.1.256 additional information TcNatC and TcNatA proteins physically interact with each other and it is plausible that this interaction takes place in the cytoplasm as suggested by their possible ribosomal cosedimentation. Subcellular localization of subunits, overview Trypanosoma cruzi
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.255 Trypanosoma cruzi Q4DSU6 AND Q4D0P4 an Esmeraldo-like catalytic NatA complex subunit ARD1/TcNaa10 and a non-Esmeraldo-like auxilliary NatA complex subunit Nat1/TcNaa15
-
2.3.1.255 Trypanosoma cruzi CL Brener Q4DSU6 AND Q4D0P4 an Esmeraldo-like catalytic NatA complex subunit ARD1/TcNaa10 and a non-Esmeraldo-like auxilliary NatA complex subunit Nat1/TcNaa15
-
2.3.1.256 Trypanosoma cruzi Q4CRN8 AND Q4DLD9 AND Q4D159 Esmeraldo-like NatC catalytic Naa30 subunit and auxiliary Naa35 subunit, and non-Esmeraldo-like auxiliary Naa38 subunit
-
2.3.1.256 Trypanosoma cruzi Q4DGZ6 AND Q4DJ45 AND Q4D159 non-Esmeraldo-like NatC catalytic Naa30 subunit and auxiliary Naa35 and Naa38 subunits
-
2.3.1.256 Trypanosoma cruzi CL Brener Q4CRN8 AND Q4DLD9 AND Q4D159 Esmeraldo-like NatC catalytic Naa30 subunit and auxiliary Naa35 subunit, and non-Esmeraldo-like auxiliary Naa38 subunit
-
2.3.1.256 Trypanosoma cruzi CL Brener Q4DGZ6 AND Q4DJ45 AND Q4D159 non-Esmeraldo-like NatC catalytic Naa30 subunit and auxiliary Naa35 and Naa38 subunits
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.255 amastigote
-
 Trypanosoma cruzi
-
2.3.1.255 epimastigote
-
 Trypanosoma cruzi
-
2.3.1.255 additional information the subunits forming the NatA enzyme complex, TcNaa10/TcNaa15, are expressed in the three main life cycle stages of the parasite, form stable complexes in vivo, and partially cosediment with the ribosome in agreement with a cotranslational function. Expression analysis of TcNaa10/TcNaa15 in the parasite Trypanosoma cruzi
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2.3.1.255 trypomastigote
-
 Trypanosoma cruzi
-
2.3.1.256 amastigote
-
 Trypanosoma cruzi
-
2.3.1.256 epimastigote
-
 Trypanosoma cruzi
-
2.3.1.256 additional information the subunits forming the NatC enzyme complex are expressed in the three main life cycle stages of the parasite, form stable complexes in vivo, and partially cosediment with the ribosome in agreement with a cotranslational function. Expression analysis of TcNaa38/TcNaa30 in the parasite Trypanosoma cruzi
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2.3.1.256 trypomastigote
-
 Trypanosoma cruzi
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.256 acetyl-CoA + N-terminal-L-methionyl-L-leucyl-glycyl-L-proline
-
 Trypanosoma cruzi N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-glycyl-L-proline + CoA
-
 ir
2.3.1.256 acetyl-CoA + N-terminal-L-methionyl-L-leucyl-glycyl-L-proline
-
 Trypanosoma cruzi CL Brener N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-glycyl-L-proline + CoA
-
 ir
2.3.1.256 additional information analysis of in vitro acetyltransferase activity of GST-tagged TcNaa30: no activity with peptides STPD, EEEIA, MDEL, and MLGP Trypanosoma cruzi ?
-
-
2.3.1.256 additional information analysis of in vitro acetyltransferase activity of GST-tagged TcNaa30: no activity with peptides STPD, EEEIA, MDEL, and MLGP Trypanosoma cruzi CL Brener ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.1.255 dimer the Trypanosoma cruzi Nat A protein complex consists of an Esmeraldo-like catalytic NatA complex subunit ARD1/TcNaa10 and a non-Esmeraldo-like auxilliary NatA complex subunit Nat1/TcNaa15 Trypanosoma cruzi
2.3.1.256 dimer the Trypanosoma cruzi Nat A protein complex consists of an Esmeraldo-like catalytic NatA complex subunit ARD1/TcNaa10 and a non-Esmeraldo-like auxilliary NatA complex subunit Nat1/TcNaa15 Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.3.1.255 ARD1
-
 Trypanosoma cruzi
2.3.1.255 NAA10
-
 Trypanosoma cruzi
2.3.1.255 NAA15
-
 Trypanosoma cruzi
2.3.1.255 NAT1
-
 Trypanosoma cruzi
2.3.1.255 NatA
-
 Trypanosoma cruzi
2.3.1.255 TcNatA
-
 Trypanosoma cruzi
2.3.1.256 NatC
-
 Trypanosoma cruzi
2.3.1.256 TcNaa30
-
 Trypanosoma cruzi
2.3.1.256 TcNaa35
-
 Trypanosoma cruzi
2.3.1.256 TcNatC
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.255 37
-
 assay at Trypanosoma cruzi
2.3.1.256 37
-
 assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.256 acetyl-CoA
-
 Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.3.1.255 additional information Trypanosoma cruzi NatA protein complex consists of one catalytic subunit and one predicted auxiliary subunit. TcNatC (EC 2.3.1.256) and TcNatA complex subunits interact in vivo and in vitro Trypanosoma cruzi
2.3.1.255 physiological function TcNatC/TcNatA proteins carry out their function independently of each other as suggested in other organisms and they may have specific functions depending on the parasite life cycle stage. But the proteins may also have other functions independent of the NAT-activity as suggested in other species Trypanosoma cruzi
2.3.1.256 additional information Trypanosoma cruzi NatC protein complex consists of one catalytic subunit TcNaa30 and one predicted auxiliary subunit TcNaa35. TcNatC and TcNatA (EC 2.3.1.255) complex subunits interact in vivo and in vitro Trypanosoma cruzi
2.3.1.256 physiological function TcNatC/TcNatA proteins carry out their function independently of each other as suggested in other organisms and they may have specific functions depending on the parasite life cycle stage. But the proteins may also have other functions independent of the NAT-activity as suggested in other species Trypanosoma cruzi