EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.4 | biotechnology | arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate | Xanthomonas campestris pv. campestris |
2.4.1.4 | synthesis | the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate | Xanthomonas campestris pv. campestris |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.4 | expression in Escherichia coli | Xanthomonas campestris pv. campestris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.4 | Ba2+ | 1.0 mM, 12% inhibition | Xanthomonas campestris pv. campestris | |
2.4.1.4 | Cu2+ | 1.0 mM, complete inhibition | Xanthomonas campestris pv. campestris | |
2.4.1.4 | Mn2+ | 1.0 mM, 50% inhibition | Xanthomonas campestris pv. campestris | |
2.4.1.4 | Ni2+ | 1.0 mM, 30% inhibition | Xanthomonas campestris pv. campestris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.4 | 9.58 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.4 | Fe2+ | 1.0 mM, 40% activation | Xanthomonas campestris pv. campestris | |
2.4.1.4 | K+ | 1.0 mM, 10% activation | Xanthomonas campestris pv. campestris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.4 | Xanthomonas campestris pv. campestris | A0A0H2X5U3 | - |
- |
2.4.1.4 | Xanthomonas campestris pv. campestris 8004 | A0A0H2X5U3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.4 | - |
Xanthomonas campestris pv. campestris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.4 | sucrose + hydroquinone | - |
Xanthomonas campestris pv. campestris | D-fructose + alpha-arbutin | - |
? | |
2.4.1.4 | sucrose + hydroquinone | - |
Xanthomonas campestris pv. campestris 8004 | D-fructose + alpha-arbutin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.4 | Amy-1 | - |
Xanthomonas campestris pv. campestris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 35 | - |
- |
Xanthomonas campestris pv. campestris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 20 | 40 | 20°C: about 60% of maximal activity, 40°C: about 70% of maximal activity | Xanthomonas campestris pv. campestris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 30 | - |
high stability, maintaining 83% activity after 2 h | Xanthomonas campestris pv. campestris |
2.4.1.4 | 50 | - |
complete loss of catalytic activity after 30 min | Xanthomonas campestris pv. campestris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.4 | 23.9 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 7.5 | - |
- |
Xanthomonas campestris pv. campestris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 5 | 8.5 | pH 5.0: 65% of maximal activity, pH 8.0: 45% of maximal activity | Xanthomonas campestris pv. campestris |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.4 | 2.5 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |