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Literature summary extracted from

  • Bashiri, G.; Nigon, L.V.; Jirgis, E.N.M.; Ho, N.A.T.; Stanborough, T.; Dawes, S.S.; Baker, E.N.; Bulloch, E.M.M.; Johnston, J.M.
    Allosteric regulation of menaquinone (vitamin K2) biosynthesis in the human pathogen Mycobacterium tuberculosis (2020), J. Biol. Chem., 295, 3759-3770 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.9 expressed in Mycobacterium smegmatis mc2 4,517 cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.9 sitting drop vapor diffusion method, using 0.1 M MOPS/HEPES, pH 7.5, 20% (v/v) glycerol, 8% (w/v) PEG 4000, and a 0.02 M mixture of sodium formate, ammonium acetate, sodium citrate tribasic dihydrate, sodium oxamate, and potassium sodium tartrate tetrahydrate Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.9 R277A the mutant has 18% of the wild type enzyme activity Mycobacterium tuberculosis
2.2.1.9 R303A the mutant has 56% of the wild type enzyme activity Mycobacterium tuberculosis
2.2.1.9 R97A the mutant has 50% of the wild type enzyme activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.9 dihydroxy-2-naphthoic acid the potent inhibitor binds to domain II of the enzyme and inhibits its activity Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.9 Mg2+ dependent on Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.9 isochorismate + 2-oxoglutarate Mycobacterium tuberculosis
-
 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate Mycobacterium tuberculosis H37Rv
-
 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.9 Mycobacterium tuberculosis
-
-
-
2.2.1.9 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.9 HisTrap column chromatography and Superdex S200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.9 isochorismate + 2-oxoglutarate
-
 Mycobacterium tuberculosis 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.9 homotetramer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.9 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase
-
 Mycobacterium tuberculosis
2.2.1.9 MenD
-
 Mycobacterium tuberculosis
2.2.1.9 Rv0555
-
 Mycobacterium tuberculosis
2.2.1.9 SEPHCHC synthase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.9 thiamine diphosphate
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.2.1.9 0.000053
-
 wild type enzyme, at pH 8.0 and 25°C Mycobacterium tuberculosis dihydroxy-2-naphthoic acid
2.2.1.9 0.00032
-
 mutant enzyme R303A, at pH 8.0 and 25°C Mycobacterium tuberculosis dihydroxy-2-naphthoic acid
2.2.1.9 0.00103
-
 mutant enzyme R97A, at pH 8.0 and 25°C Mycobacterium tuberculosis dihydroxy-2-naphthoic acid