EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.15 | expressed in Escherichia coli strain HBlOl or MC4100 | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.4.15 | periplasm | - |
Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.15 | Mg2+ | 1 mM used in assay conditions | Escherichia coli | |
1.8.4.15 | Zn2+ | 0.16 mM used in assay conditions | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.4.15 | 40000 | 50000 | gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.15 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.15 | DEAE-Sepharose CL-GB column chromatography and Sephacryl S-200 gel filtration | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.15 | dithiothreitol + reduced bovine ribonuclease A | - |
Escherichia coli | ? + oxidized bovine ribonuclease A | - |
? | |
1.8.4.15 | GSH + scrambled bovine ribonuclease A | - |
Escherichia coli | ? | - |
? | |
1.8.4.15 | GSSG + reduced alkaline phosphatase | - |
Escherichia coli | 2 GSH + oxidized alkaline phosphatase | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.4.15 | homodimer | x * 21000, SDS-PAGE | Escherichia coli |
1.8.4.15 | homodimer | x * 21132, calculated from amino acid sequence | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.15 | DsbA | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.4.15 | physiological function | the enzyme directly catalyzes disulfide bond formation and also has a role in maintaining the bacterial periplasm oxidative | Escherichia coli |