Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hirata, A.; Suzuki, T.; Nagano, T.; Fujii, D.; Okamoto, M.; Sora, M.; Lowe, T.M.; Kanai, T.; Atomi, H.; Suzuki, T.; Hori, H.
    Distinct modified nucleosides in tRNATrp from thehyperthermophilic archaeon Thermococcus kodakarensis and requirement of tRNA m2G10/m22G10 methyltransferase (archaeal Trm11) for survival at high temperatures (2019), J. Bacteriol., 201, e00448-19 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.214 gene Tk0981 Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.214 additional information construction of a gene disruptant trm11 (DELTAtrm11) mutant showing the absence of m2s2G10. The lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggests that this methylation is mediated by Trm14, which is an m2G6 methyltransferase. The trm11 gene is reinserted into the chiA (Tk1765, chitinase gene) region in the genomic DNA of the DELTAtrm11 strain. Deletion of the Tk1765 gene does not cause growth defects unless chitin is used as a carbon source. Although its expression level is lower in the complemented strain than in the wild-type strain, Trm11 is expressed in the complemented strain. At 85°C, the wild-type, DELTAtrm11, and complemented strains show similar growth curves. As the temperature increases, the growth of the DELTAtrm11 strain is clearly slower than that of the wild-type or complemented strain. At 95°C, the DELTAtrm11 strain shows a considerable growth defect, whereas the complemented strain grows at approximately the same speed as the wild-type strain, indicating that the growth defect of the DELTAtrm11 strain is due to the lack of archaeal Trm11 protein Thermococcus kodakarensis
2.1.1.256 additional information construction of a trm11 trm14 double disruptant strain that shows a lack of 2-methylguanosine (m2G) at position 67 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA Thermococcus kodakarensis
-
 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA Thermococcus kodakarensis JCM 12380
-
 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA Thermococcus kodakarensis ATCC BAA-918
-
 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine67 in tRNATrp Thermococcus kodakarensis
-
 S-adenosyl-L-homocysteine + N2-methylguanine67 in tRNATrp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.214 Thermococcus kodakarensis Q5JID5 i.e. Pyrococcus kodakaraensis strain KOD1
-
2.1.1.214 Thermococcus kodakarensis ATCC BAA-918 Q5JID5 i.e. Pyrococcus kodakaraensis strain KOD1
-
2.1.1.214 Thermococcus kodakarensis JCM 12380 Q5JID5 i.e. Pyrococcus kodakaraensis strain KOD1
-
2.1.1.256 Thermococcus kodakarensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.214 additional information hyperthermophilic archaeon Thermococcus kodakarensis grows at 60-100°C Thermococcus kodakarensis
-
2.1.1.256 additional information hyperthermophilic archaeon Thermococcus kodakarensis grows at 60-100°C Thermococcus kodakarensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA
-
 Thermococcus kodakarensis JCM 12380 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNA
-
 Thermococcus kodakarensis ATCC BAA-918 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNATrp
-
 Thermococcus kodakarensis JCM 12380 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp
-
 ?
2.1.1.214 S-adenosyl-L-methionine + guanine10 in tRNATrp
-
 Thermococcus kodakarensis ATCC BAA-918 S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine37 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine37 in tRNATrp
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine54 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine54 in tRNATrp
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine67 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine67 in tRNATrp
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine8 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine8 in tRNATrp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.214 archaeal Trm11
-
 Thermococcus kodakarensis
2.1.1.214 N2, N2-dimethylguanosine tRNA methyltransferase UniProt Thermococcus kodakarensis
2.1.1.214 N2,N2-dimethylguanosine tRNA methyltransferase
-
 Thermococcus kodakarensis
2.1.1.214 Tk0981
-
 Thermococcus kodakarensis
2.1.1.214 Trm-G10
-
 Thermococcus kodakarensis
2.1.1.214 Trm-m22G10
-
 Thermococcus kodakarensis
2.1.1.214 Trm11
-
 Thermococcus kodakarensis
2.1.1.214 tRNA m2G10/m22G10 methyltransferase
-
 Thermococcus kodakarensis
2.1.1.256 m2G6 methyltransferase
-
 Thermococcus kodakarensis
2.1.1.256 Trm14
-
 Thermococcus kodakarensis
2.1.1.256 tRNA m2G6 methyltransferase
-
 Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.214 additional information although the eukaryotic counterpart (Trm11) requires another subunit Trm112 for enzymatic activity, the archaeal enzyme does not require a partner subunit Thermococcus kodakarensis
2.1.1.214 S-adenosyl-L-methionine
-
 Thermococcus kodakarensis
2.1.1.256 S-adenosyl-L-methionine
-
 Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
2.1.1.214 evolution although the eukaryotic counterpart (Trm11) requires another subunit Trm112 for enzymatic activity, the archaeal enzyme does not require a partner subunit. Furthermore, the eukaryotic Trm11-Trm112 complex catalyzes a single methyl transfer reaction and forms only m2G10 in tRNA. The archaeal enzyme is called Trm-G10 or Trm-m22G10 to distinguish it from the eukaryotic enzyme Thermococcus kodakarensis
2.1.1.214 malfunction the DELTAtrm11 knockout strain grows poorly at 95°C Thermococcus kodakarensis
2.1.1.214 physiological function tRNA m2G10/m2 2G10 methyltransferase (archaeal Trm11) methylates the 2-amino group in guanosine at position 10 in tRNA and forms N2,N2-dimethylguanosine (m22G10) via intermediate N2-methylguanosine (m2G10). The archaeal Trm11 is required for Thermococcus kodakarensis survival at high temperatures. The m22G10 modification might have effects on stabilization of tRNA and/or correct folding of tRNA at the high temperatures Thermococcus kodakarensis
2.1.1.256 evolution a distinct tRNA 2'-O-methyltransferase, which methylates the 2'-OH of ribose at position 6 in tRNA and does not differentiate between adenine and cytosine, may exist in Thermococcus and Pyrococcus genera. In terms of the other enzymes responsible for the observed 2'-O-methylations, Cm56 is a product of Trm56 (EC 2.1.1.206) Thermococcus kodakarensis
2.1.1.256 malfunction the lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggests that this methylation is mediated by m2G6 methyltransferase Trm14 Thermococcus kodakarensis
2.1.1.256 physiological function the trm14 gene is responsible for the m2G67 modification. The modification site (G67) forms a Watson-Crick base pair with C6 in tRNA. Archaeal Trm14 methylates G6 in tRNA and contains a THUMP domain, which often recognizes the CCA terminus in tRNA. But it has not been confirmed that Thermococcus kodakarensis Trm14 methylates G6 in tRNA like Methanocaldococcus jannaschii Trm14. Furthermore, the presence of m2G6 modification in Thermococcus kodakarensis tRNAs is not confirmed. In total, trm14 is responsible for m2G at positions 8, 54, 37, and 67, respectively, as determined by analysis of tRNATrp from gene disruptant strains Thermococcus kodakarensis