EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.173 | additional information | ATP (0.01 to 1 mM), ADP (0.01 to 0.1 mM), and coenzyme A (0.5 mM) do not enhance activity of the purified enzyme | Cupriavidus sp. GP1 |
EC Number | General Stability | Organism |
---|---|---|
1.14.14.173 | dithiothreitol (0.5 mM), glycerol (10% (v/v)), sucrose (10% (w/v)), or bovine liver catalase (30 ng/ml), separately or in combinations, slightly enhance enzyme stability. No influence on stability is found for 10% (v/v) ethanol, isopropanol, or H2O2 (0.1 mM). FAD (0.03 mM) leads to a slight destabilization | Cupriavidus sp. GP1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.173 | 2,4,6-Trichlorophenol | at 2,4,6-trichlorophenol concentrations higher than 0.1 mM, substrate inhibition which amounts to 43% at 0.2 mM 2,4,6-trichlorophenol and to 72% at 0.33 mM 2,4,6-trichlorophenol is observed | Cupriavidus sp. GP1 | |
1.14.14.173 | 4-hydroxymercuribenzoate | 62% inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Acriflavine | complete inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Cu2+ | complete inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Hg2+ | 87% inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | additional information | not inhibited by menadione, metapyrone, EDTA, Tiron, 1,10-phenanthroline, KCN and sodium azide (1 mM each) | Cupriavidus sp. GP1 | |
1.14.14.173 | N-ethylmaleimide | 42% inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Quinacrine | complete inhibition at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Zn2+ | 31% inhibition at 0.1 mM | Cupriavidus sp. GP1 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.173 | Co2+ | slight activation at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | Fe2+ | slight activation at 0.1 mM. The enzyme contains 0.2 mol of Fe per mol of the homotetrameric protein | Cupriavidus sp. GP1 | |
1.14.14.173 | Fe3+ | slight activation at 0.1 mM | Cupriavidus sp. GP1 | |
1.14.14.173 | additional information | Fe2+ (0.2 mM) and Mg2+ (0.8 mM) do not enhance activity of the purified enzyme. No influence on enzyme activity is observed for 1 mM Mg2+, Ca2+, and Co2+ | Cupriavidus sp. GP1 | |
1.14.14.173 | Ni2+ | slight activation at 0.1 mM | Cupriavidus sp. GP1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.173 | 240000 | - |
gel filtration | Cupriavidus sp. GP1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | Cupriavidus sp. GP1 | - |
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | Cupriavidus sp. GP1 DSM 6428 | - |
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | Cupriavidus sp. GP1 ATCC 49806 | - |
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | Cupriavidus sp. GP1 | - |
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | Cupriavidus sp. GP1 DSM 6428 | - |
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | Cupriavidus sp. GP1 ATCC 49806 | - |
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.173 | Cupriavidus sp. GP1 | - |
- |
- |
1.14.14.173 | Cupriavidus sp. GP1 ATCC 49806 | - |
- |
- |
1.14.14.173 | Cupriavidus sp. GP1 DSM 6428 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.173 | DEAE-cellulose column chromatography, DE52 cellulose anion-exchange chromatography, and Resource Q anion -exchange chromatography | Cupriavidus sp. GP1 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.173 | 0.18 | - |
crude extract, pH and temperature not specified in the publication | Cupriavidus sp. GP1 |
1.14.14.173 | 6.87 | - |
after 38.2fold purification, pH and temperature not specified in the publication | Cupriavidus sp. GP1 |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.14.173 | -20 C, the purified enzyme is stable for several months | Cupriavidus sp. GP1 |
1.14.14.173 | 25 C, purified enzyme, 8-15 h, complete loss of activity | Cupriavidus sp. GP1 |
1.14.14.173 | 4 C, purified enzyme, 2 to 3 days, complete loss of activity | Cupriavidus sp. GP1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.173 | 2,3,4,5-tetrachlorophenol + FADH2 + O2 | 33% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,3,4,5-tetrachlorophenol + FADH2 + O2 | 33% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 DSM 6428 | ? | - |
? | |
1.14.14.173 | 2,3,4,5-tetrachlorophenol + FADH2 + O2 | 33% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 ATCC 49806 | ? | - |
? | |
1.14.14.173 | 2,3,4,6-tetrachlorophenol + FADH2 + O2 | 67% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,4,5-trichlorophenol + FADH2 + O2 | 33% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,4,6-tribromophenol + FADH2 + O2 | 57% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | - |
Cupriavidus sp. GP1 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | highest conversion rate | Cupriavidus sp. GP1 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | - |
Cupriavidus sp. GP1 DSM 6428 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | highest conversion rate | Cupriavidus sp. GP1 DSM 6428 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | - |
Cupriavidus sp. GP1 ATCC 49806 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-trichlorophenol + FADH2 + O2 | highest conversion rate | Cupriavidus sp. GP1 ATCC 49806 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,4,6-triiodophenol + FADH2 + O2 | 50% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,4-dichlorophenol + FADH2 + O2 | 80% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | - |
Cupriavidus sp. GP1 | 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | - |
Cupriavidus sp. GP1 DSM 6428 | 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorohydroquinone + FADH2 + O2 | - |
Cupriavidus sp. GP1 ATCC 49806 | 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorophenol + FADH2 + O2 | 9% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | 2,6-dichlorohydroquinone + FAD + H2O | - |
? | |
1.14.14.173 | 2,6-dichlorophenol + FADH2 + O2 | 67% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 2-chlorophenol + FADH2 + O2 | 25% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 3,4,5-trichlorophenol + FADH2 + O2 | 25% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 3,4-dichlorophenol + FADH2 + O2 | 50% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | 4-chlorophenol + FADH2 + O2 | 32% activity compared to 2,4,6-trichlorophenol | Cupriavidus sp. GP1 | ? | - |
? | |
1.14.14.173 | additional information | no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol | Cupriavidus sp. GP1 | ? | - |
- |
|
1.14.14.173 | additional information | no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol | Cupriavidus sp. GP1 DSM 6428 | ? | - |
- |
|
1.14.14.173 | additional information | no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol | Cupriavidus sp. GP1 ATCC 49806 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.173 | homotetramer | 4 * 60000, SDS-PAGE | Cupriavidus sp. GP1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.173 | 2,4,6-trichlorophenol-4-monooxygenase | - |
Cupriavidus sp. GP1 |
1.14.14.173 | TCP-4-monooxygenase | - |
Cupriavidus sp. GP1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.173 | 35 | 40 | - |
Cupriavidus sp. GP1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.173 | 7 | - |
optimum activity in 25 to 50 mM Tris-HCl buffer, pH 7.0 | Cupriavidus sp. GP1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.173 | FAD | 0.03 mM FAD leads to a 4-5fold activation of the DEAE-cellulose-purified enzyme. FAD cannot be replaced by flavin mononucleotide or riboflavin | Cupriavidus sp. GP1 | |
1.14.14.173 | NADH | the activity with NADH is about 20fold higher than with NADPH | Cupriavidus sp. GP1 |