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Literature summary extracted from

  • Park, S.H.; Suh, S.W.; Song, H.K.
    A cytosine modification mechanism revealed by the structure of a ternary complex of deoxycytidylate hydroxymethylase from bacteriophage T4 with its cofactor and substrate (2019), IUCrJ, 6, 206-217 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 expressed in Escherichia coli BL21(DE3) cells Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.8 C148S/D179N mutant in complex with dCMP and tetrahydrofolate, hanging drop vapor diffusion method, using 100 mM Tris-HCl pH 8.5 and 0.96 M sodium citrate Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.8 C148S/D179N active site mutant Tequatrovirus T4
2.1.2.8 D145N the mutant shows no activity Tequatrovirus T4
2.1.2.8 D179N the mutation significantly decreases the binding affinity to dCMP Tequatrovirus T4
2.1.2.8 S94A the mutant 25% residual activity compared to the wild type enzyme Tequatrovirus T4
2.1.2.8 S94N the mutant shows no activity Tequatrovirus T4
2.1.2.8 Y96F the mutant shows no activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + dCMP Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyl-dCMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4 P08773
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.8 HisTrap column chromatography and Superdex 200 gel filtration Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + dCMP
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyl-dCMP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.8 DCH
-
Tequatrovirus T4
2.1.2.8 dCMP hydroxymethylase
-
Tequatrovirus T4
2.1.2.8 deoxycytidylate hydroxymethylase
-
Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methenyltetrahydrofolate
-
Tequatrovirus T4