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Literature summary extracted from

  • Khajehzadeh, M.; Mehrnejad, F.; Pazhang, M.; Doustdar, F.
    Effects of sorbitol and glycerol on the structure, dynamics, and stability of Mycobacterium tuberculosis pyrazinamidase (2016), Int. J. Mycobacteriol., 5 Suppl 1, S138-S139 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.B15 glycerol increases the enzyme activity up to 130% at 37°C, gives rise to an increase in the content of alpha-helix and beta-sheets elements. More stability of PZase in glycerol solution correlates with its amphiphilic orientation, which decreases the unfavorable interactions of hydrophobic regions. Mycobacterium tuberculosis
3.5.1.B15 sorbitol increases the enzyme activity up to 110% at 37°C, gives rise to an increase in the content of alpha-helix and beta-sheets elements Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.B15 recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.B15 pyrazinamide + H2O Mycobacterium tuberculosis
-
 pyrazinoic acid + NH3
-
 ?
3.5.1.B15 pyrazinamide + H2O Mycobacterium tuberculosis H37Rv
-
 pyrazinoic acid + NH3
-
 ?
3.5.1.B15 pyrazinamide + H2O Mycobacterium tuberculosis ATCC 25618
-
 pyrazinoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.B15 Mycobacterium tuberculosis I6XD65
-
-
3.5.1.B15 Mycobacterium tuberculosis ATCC 25618 I6XD65
-
-
3.5.1.B15 Mycobacterium tuberculosis H37Rv I6XD65
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.B15 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.B15 pyrazinamide + H2O
-
 Mycobacterium tuberculosis pyrazinoic acid + NH3
-
 ?
3.5.1.B15 pyrazinamide + H2O
-
 Mycobacterium tuberculosis H37Rv pyrazinoic acid + NH3
-
 ?
3.5.1.B15 pyrazinamide + H2O
-
 Mycobacterium tuberculosis ATCC 25618 pyrazinoic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.B15 PncA
-
 Mycobacterium tuberculosis
3.5.1.B15 PZAse
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.B15 37
-
 assay at Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.1.B15 malfunction pyrazinamide (PZA)-resistance in Mycobacterium tuberculosis strains is caused by point mutations in the PZase enzyme which is the activator of the prodrug pyrazinoic acid (PZA) Mycobacterium tuberculosis
3.5.1.B15 additional information structure analysis by molecular dynamics (MD) simulations, and spectroscopic methods, such as fluorescence spectroscopy and circular dichroism (CD) Mycobacterium tuberculosis
3.5.1.B15 physiological function pyrazinamidase (PZase) is involved in degradation of pyrazinamide to ammonia and pyrazinoic acid Mycobacterium tuberculosis