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Literature summary extracted from

  • Hayes, R.P.; Webb, B.N.; Subramanian, A.K.; Nissen, M.; Popchock, A.; Xun, L.; Kang, C.
    Structural and catalytic differences between two FADH2-dependent monooxygenases 2,4,5-TCP 4-monooxygenase (TftD) from Burkholderia cepacia AC1100 and 2,4,6-TCP 4-monooxygenase (TcpA) from Cupriavidus necator JMP134 (2012), Int. J. Mol. Sci., 13, 9769-9784 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.173 expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.173 hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 (pH 7.4) with 0.2 M potassium sodium tartrate tetrahydrate Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator JMP134
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator JMP134
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.173 Cupriavidus necator Q8KTD8
-
-
1.14.14.173 Cupriavidus necator JMP134 Q8KTD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.173 Ni-NTA column chromatography and Mono Q column chromatography Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator JMP134 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator JMP134 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.173 2,4,6-TCP 4-monooxygenase
-
 Cupriavidus necator
1.14.14.173 tcpA
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.173 FADH2
-
 Cupriavidus necator