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Literature summary extracted from

  • Li, W.; Qiao, C.; Pang, J.; Zhang, G.; Luo, Y.
    The versatile O-methyltransferase LrOMT catalyzes multiple O-methylation reactions in amaryllidaceae alkaloids biosynthesis (2019), Int. J. Biol. Macromol., 141, 680-692 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.336 overexpression in Escherichia coli Lycoris radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.336 0.12
-
caffeic acid pH 9.0, 37°C, product: isoferulic acid Lycoris radiata
2.1.1.336 0.225
-
caffeic acid pH 9.0, 37°C, product: ferulic acid Lycoris radiata
2.1.1.336 0.251
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: isovanillin Lycoris radiata
2.1.1.336 0.267
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: vanillin Lycoris radiata
2.1.1.336 0.294
-
norbelladine pH 9.0, 37°C, product: 4'-O-methylnorbelladine Lycoris radiata
2.1.1.336 0.396
-
norbelladine pH 9.0, 37°C, product: 3'-O-methylnorbelladine Lycoris radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.336 2 S-adenosyl-L-methionine + norbelladine Lycoris radiata the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-Omethylnorbelladine 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.336 Lycoris radiata A0A5C2H154
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.336
-
Lycoris radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.336 2 adenosyl-L-methionine + 1,2,3,4-4H-6,7-isoquinolinediol 1,2,3,4-4H-6-methoxy-7-isoquinolinol and 1,2,3,4-4H-7-methoxy-6-isoquinolinol are produced in the ratio 10.5:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 1,2,3,4-4H-6-methoxy-7-isoquinolinol + 1,2,3,4-4H-7-methoxy-6-isoquinolinol
-
?
2.1.1.336 2 S-adenosyl-L-methionine + (-)-epinephrine 4-O-methylepinephrine and 3-O-methylepinephrine are produced in the ratio 6.0:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 4-O-methylepinephrine + 3-O-methylepinephrine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + (-)-norepinephrine 4-O-methylnorepinephrine and 3-O-methylnorepinephrine are produced in the ratio 14.7:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 4-O-methylnorepinephrine + 3-O-methylnorepinephrine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 3,4,5-trihydroxybenzaldehyde 3,5-dihydroxy-4-methoxybenzaldehyde and 5-hydroxyvanillin are produced in the ratio 1:99.9 Lycoris radiata 2 S-adenosyl-L-homocysteine + 3,5-dihydroxy-4-methoxybenzaldehyde + 5-hydroxyvanillin
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde vanillin and isovanillin are produced in the ratio 1:10.5 Lycoris radiata 2 S-adenosyl-L-homocysteine + vanillin + isovanillin
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 3,4-dihydroxybenzylamine 3-hydroxy-4-methoxybenzylamine and 4-hydroxy-3-methoxybenzylamine are produced in the ratio 21.4:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 3-hydroxy-4-methoxybenzylamine + 4-hydroxy-3-methoxybenzylamine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 4-bromo-catechol 5-bromo-2-methoxyphenol and 4-bromo-2-methoxyphenol are produced in the ratio 54.8:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 5-bromo-2-methoxyphenol + 4-bromo-2-methoxyphenol
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 4-fluoro-catechol 5-fluoro-2-methoxyphenol and 4-fluoro-2-methoxyphenol are produced in the ratio 1.5:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 5-fluoro-2-methoxyphenol + 4-fluoro-2-methoxyphenol
-
?
2.1.1.336 2 S-adenosyl-L-methionine + 5-hydroxyvanillin 3,4-dimethoxy-5-hydroxybenzaldehyde and 3,5-dimethoxy-4-hydroxybenzaldehyde are produced in the ratio 1:27.9 Lycoris radiata 2 S-adenosyl-L-homocysteine + 3,4-dimethoxy-5-hydroxybenzaldehyde + 3,5-dimethoxy-4-hydroxybenzaldehyde
-
?
2.1.1.336 2 S-adenosyl-L-methionine + caffeic acid ferulic acid and isoferulic acid are produced in the ratio 1:1.3 Lycoris radiata 2 S-adenosyl-L-homocysteine + ferulic acid + isoferulic acid
-
?
2.1.1.336 2 S-adenosyl-L-methionine + dopamine 4-O-methyldopamine and 3-O-methyldopamine are produced in the ratio 83.8:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 4-O-methyldopamine + 3-O-methyldopamine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + ethyl 3,4-dihydroxybenzoate ethyl 3-hydroxy-4-methoxybenzoate and ethyl 4-hydroxy-3-methoxybenzoate are produced in the ratio 1:99.9 Lycoris radiata 2 S-adenosyl-L-homocysteine + ethyl 3-hydroxy-4-methoxybenzoate + ethyl 4-hydroxy-3-methoxybenzoate
-
?
2.1.1.336 2 S-adenosyl-L-methionine + higenamine coclaurine and isococlaurine are produced in the ratio 99.9:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + coclaurine + isococlaurine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + norbelladine the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-Omethylnorbelladine Lycoris radiata 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine
-
?
2.1.1.336 2 S-adenosyl-L-methionine + norbelladine the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-O-methylnorbelladine. 4'-O-Methylnorbelladine and 3'-O-methylnorbelladine in the ratio of 3.5:1 Lycoris radiata 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.336 N4OMT
-
Lycoris radiata
2.1.1.336 norbelladine 4'-O-methyltransferase
-
Lycoris radiata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.336 0.0011
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: isovanillin Lycoris radiata
2.1.1.336 0.0023
-
caffeic acid pH 9.0, 37°C, product: isoferulic acid Lycoris radiata
2.1.1.336 0.00275
-
norbelladine pH 9.0, 37°C, product: 3'-O-methylnorbelladine Lycoris radiata
2.1.1.336 0.0032
-
caffeic acid pH 9.0, 37°C, product: ferulic acid Lycoris radiata
2.1.1.336 0.0084
-
norbelladine pH 9.0, 37°C, product: 4'-O-methylnorbelladine Lycoris radiata
2.1.1.336 0.0179
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: vanillin Lycoris radiata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.336 0.00438
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: isovanillin Lycoris radiata
2.1.1.336 0.0069
-
norbelladine pH 9.0, 37°C, product: 3'-O-methylnorbelladine Lycoris radiata
2.1.1.336 0.01435
-
caffeic acid pH 9.0, 37°C, product: ferulic acid Lycoris radiata
2.1.1.336 0.0194
-
caffeic acid pH 9.0, 37°C, product: isoferulic acid Lycoris radiata
2.1.1.336 0.0285
-
norbelladine pH 9.0, 37°C, product: 4'-O-methylnorbelladine Lycoris radiata
2.1.1.336 0.0673
-
3,4-Dihydroxybenzaldehyde pH 9.0, 37°C, product: vanillin Lycoris radiata