EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.336 | overexpression in Escherichia coli | Lycoris radiata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.336 | 0.12 | - |
caffeic acid | pH 9.0, 37°C, product: isoferulic acid | Lycoris radiata | |
2.1.1.336 | 0.225 | - |
caffeic acid | pH 9.0, 37°C, product: ferulic acid | Lycoris radiata | |
2.1.1.336 | 0.251 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: isovanillin | Lycoris radiata | |
2.1.1.336 | 0.267 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: vanillin | Lycoris radiata | |
2.1.1.336 | 0.294 | - |
norbelladine | pH 9.0, 37°C, product: 4'-O-methylnorbelladine | Lycoris radiata | |
2.1.1.336 | 0.396 | - |
norbelladine | pH 9.0, 37°C, product: 3'-O-methylnorbelladine | Lycoris radiata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.336 | 2 S-adenosyl-L-methionine + norbelladine | Lycoris radiata | the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-Omethylnorbelladine | 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.336 | Lycoris radiata | A0A5C2H154 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.336 | - |
Lycoris radiata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.336 | 2 adenosyl-L-methionine + 1,2,3,4-4H-6,7-isoquinolinediol | 1,2,3,4-4H-6-methoxy-7-isoquinolinol and 1,2,3,4-4H-7-methoxy-6-isoquinolinol are produced in the ratio 10.5:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 1,2,3,4-4H-6-methoxy-7-isoquinolinol + 1,2,3,4-4H-7-methoxy-6-isoquinolinol | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + (-)-epinephrine | 4-O-methylepinephrine and 3-O-methylepinephrine are produced in the ratio 6.0:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 4-O-methylepinephrine + 3-O-methylepinephrine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + (-)-norepinephrine | 4-O-methylnorepinephrine and 3-O-methylnorepinephrine are produced in the ratio 14.7:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 4-O-methylnorepinephrine + 3-O-methylnorepinephrine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 3,4,5-trihydroxybenzaldehyde | 3,5-dihydroxy-4-methoxybenzaldehyde and 5-hydroxyvanillin are produced in the ratio 1:99.9 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 3,5-dihydroxy-4-methoxybenzaldehyde + 5-hydroxyvanillin | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | vanillin and isovanillin are produced in the ratio 1:10.5 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + vanillin + isovanillin | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 3,4-dihydroxybenzylamine | 3-hydroxy-4-methoxybenzylamine and 4-hydroxy-3-methoxybenzylamine are produced in the ratio 21.4:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 3-hydroxy-4-methoxybenzylamine + 4-hydroxy-3-methoxybenzylamine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 4-bromo-catechol | 5-bromo-2-methoxyphenol and 4-bromo-2-methoxyphenol are produced in the ratio 54.8:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 5-bromo-2-methoxyphenol + 4-bromo-2-methoxyphenol | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 4-fluoro-catechol | 5-fluoro-2-methoxyphenol and 4-fluoro-2-methoxyphenol are produced in the ratio 1.5:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 5-fluoro-2-methoxyphenol + 4-fluoro-2-methoxyphenol | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + 5-hydroxyvanillin | 3,4-dimethoxy-5-hydroxybenzaldehyde and 3,5-dimethoxy-4-hydroxybenzaldehyde are produced in the ratio 1:27.9 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 3,4-dimethoxy-5-hydroxybenzaldehyde + 3,5-dimethoxy-4-hydroxybenzaldehyde | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + caffeic acid | ferulic acid and isoferulic acid are produced in the ratio 1:1.3 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + ferulic acid + isoferulic acid | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + dopamine | 4-O-methyldopamine and 3-O-methyldopamine are produced in the ratio 83.8:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 4-O-methyldopamine + 3-O-methyldopamine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + ethyl 3,4-dihydroxybenzoate | ethyl 3-hydroxy-4-methoxybenzoate and ethyl 4-hydroxy-3-methoxybenzoate are produced in the ratio 1:99.9 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + ethyl 3-hydroxy-4-methoxybenzoate + ethyl 4-hydroxy-3-methoxybenzoate | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + higenamine | coclaurine and isococlaurine are produced in the ratio 99.9:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + coclaurine + isococlaurine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + norbelladine | the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-Omethylnorbelladine | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine | - |
? | |
2.1.1.336 | 2 S-adenosyl-L-methionine + norbelladine | the enzyme exhibis both para- and meta-O-methylation activities toward norbelladine to give 4'- and 3'-O-methylnorbelladine. 4'-O-Methylnorbelladine and 3'-O-methylnorbelladine in the ratio of 3.5:1 | Lycoris radiata | 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.336 | N4OMT | - |
Lycoris radiata |
2.1.1.336 | norbelladine 4'-O-methyltransferase | - |
Lycoris radiata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.336 | 0.0011 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: isovanillin | Lycoris radiata | |
2.1.1.336 | 0.0023 | - |
caffeic acid | pH 9.0, 37°C, product: isoferulic acid | Lycoris radiata | |
2.1.1.336 | 0.00275 | - |
norbelladine | pH 9.0, 37°C, product: 3'-O-methylnorbelladine | Lycoris radiata | |
2.1.1.336 | 0.0032 | - |
caffeic acid | pH 9.0, 37°C, product: ferulic acid | Lycoris radiata | |
2.1.1.336 | 0.0084 | - |
norbelladine | pH 9.0, 37°C, product: 4'-O-methylnorbelladine | Lycoris radiata | |
2.1.1.336 | 0.0179 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: vanillin | Lycoris radiata |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.336 | 0.00438 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: isovanillin | Lycoris radiata | |
2.1.1.336 | 0.0069 | - |
norbelladine | pH 9.0, 37°C, product: 3'-O-methylnorbelladine | Lycoris radiata | |
2.1.1.336 | 0.01435 | - |
caffeic acid | pH 9.0, 37°C, product: ferulic acid | Lycoris radiata | |
2.1.1.336 | 0.0194 | - |
caffeic acid | pH 9.0, 37°C, product: isoferulic acid | Lycoris radiata | |
2.1.1.336 | 0.0285 | - |
norbelladine | pH 9.0, 37°C, product: 4'-O-methylnorbelladine | Lycoris radiata | |
2.1.1.336 | 0.0673 | - |
3,4-Dihydroxybenzaldehyde | pH 9.0, 37°C, product: vanillin | Lycoris radiata |