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Literature summary extracted from

  • Kumar, A.; Wu, G.; Wu, Z.; Kumar, N.; Liu, Z.
    Improved catalytic properties of a serine hydroxymethyl transferase from Idiomarina loihiensis by site directed mutagenesis (2018), Int. J. Biol. Macromol., 117, 1216-1223 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Idiomarina loihiensis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 G132P the mutant shows 301% activity compared to the wild type enzyme Idiomarina loihiensis
2.1.2.1 H61G the mutant shows 283% activity compared to the wild type enzyme Idiomarina loihiensis
2.1.2.1 H61G/G132P the mutant shows 356% activity compared to the wild type enzyme Idiomarina loihiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.27
-
 DL-phenylserine wild type enzyme, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 81.9
-
 DL-phenylserine mutant enzyme G132P, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 93.2
-
 DL-phenylserine mutant enzyme H61G/G132P, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 131.6
-
 DL-phenylserine mutant enzyme H61G, at pH 7.0 and 37°C Idiomarina loihiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Idiomarina loihiensis
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Idiomarina loihiensis
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Idiomarina loihiensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 glutathione-Sepharose resin column chromatography and Superdex S200 gel filtration Idiomarina loihiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Idiomarina loihiensis tetrahydrofolate + L-serine
-
 r
2.1.2.1 DL-phenylserine + ?
-
 Idiomarina loihiensis benzaldehyde + ?
-
 ?
2.1.2.1 tetrahydrofolate + L-serine
-
 Idiomarina loihiensis 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 ? x * 45400, SDS-PAGE and calculated from amino acid sequence Idiomarina loihiensis

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 GlyA
-
 Idiomarina loihiensis
2.1.2.1 serine hydroxymethyl transferase
-
 Idiomarina loihiensis
2.1.2.1 SHMT
-
 Idiomarina loihiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 50
-
-
 Idiomarina loihiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.2.1 35 60 more than 45% activity between 35 and 60°C Idiomarina loihiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 45 65 the wild type enzyme shows a rapid decrease (to less than 20%) in activity after 30 min at 55-65°C, and shows about 35% activity after 30 min at 45°C Idiomarina loihiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 1.51
-
 DL-phenylserine wild type enzyme, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 2.34
-
 DL-phenylserine mutant enzyme H61G, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 3.67
-
 DL-phenylserine mutant enzyme G132P, at pH 7.0 and 37°C Idiomarina loihiensis
2.1.2.1 6.89
-
 DL-phenylserine mutant enzyme H61G/G132P, at pH 7.0 and 37°C Idiomarina loihiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7.5
-
-
 Idiomarina loihiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 7 10 about 20% activity at pH 6.5, about 85% activity at pH 7.0, about 95% activity at pH 8.0, about 75% activity at pH 9.0, about 45% activity at pH 10.0 Idiomarina loihiensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Idiomarina loihiensis