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Literature summary extracted from

  • Johnston, D.M.; Miot, M.; Hoskins, J.R.; Wickner, S.; Doyle, S.M.
    Substrate discrimination by ClpB and Hsp104 (2017), Front. Mol. Biosci., 4, 36 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.10 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus
3.6.4.10 expressed in Escherichia coli strain Rosetta(DE3) Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.10 ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
 ?
3.6.4.10 ATP + H2O Thermus thermophilus
-
 ADP + phosphate
-
 ?
3.6.4.10 additional information Thermus thermophilus the enzyme collaborates with the DnaK chaperone system to dissolve protein aggregates both in vivo and in vitro ?
-
-
3.6.4.10 additional information Saccharomyces cerevisiae the enzyme collaborates with the Hsp70 chaperone system to dissolve protein aggregates both in vivo and in vitro ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.10 Saccharomyces cerevisiae
-
-
-
3.6.4.10 Thermus thermophilus Q9RA63
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.10 Q-Sepharose column chromatography and Sephacryl S-200 gel filtration Saccharomyces cerevisiae
3.6.4.10 Q-Sepharose column chromatography and Sephacryl S-200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.10 ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
3.6.4.10 ATP + H2O
-
 Thermus thermophilus ADP + phosphate
-
 ?
3.6.4.10 additional information the enzyme collaborates with the DnaK chaperone system to dissolve protein aggregates both in vivo and in vitro Thermus thermophilus ?
-
-
3.6.4.10 additional information the enzyme collaborates with the Hsp70 chaperone system to dissolve protein aggregates both in vivo and in vitro Saccharomyces cerevisiae ?
-
-
3.6.4.10 additional information the enzyme unfolds GFP-X30-H6,GFP-X7-H6, and GFP-15 with Km values of 0.0018 mM, 0.0052 mM, and 0.0016 mM, respectively Saccharomyces cerevisiae ?
-
-
3.6.4.10 additional information the enzyme unfolds GFP-X30-H6,GFP-X7-H6, and GFP-15 with Km values of 0.0041 mM, 0.003 mM, and 0.0012 mM, respectively Thermus thermophilus ?
-
-

Subunits

EC Number Subunits Comment Organism
3.6.4.10 hexamer
-
 Saccharomyces cerevisiae
3.6.4.10 hexamer
-
 Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.6.4.10 ClpB
-
 Thermus thermophilus
3.6.4.10 Hsp104
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
3.6.4.10 physiological function the enzyme is required for thermotolerance and function in disaggregation and reactivation of aggregated proteins that form during severe stress conditions Saccharomyces cerevisiae
3.6.4.10 physiological function the enzyme is required for thermotolerance and function in disaggregation and reactivation of aggregated proteins that form during severe stress conditions Thermus thermophilus