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Literature summary extracted from

  • Mazmouz, R.; Chapuis-Hugon, F.; Pichon, V.; Mejean, A.; Ploux, O.
    The last step of the biosynthesis of the cyanotoxins cylindrospermopsin and 7-epi-cylindrospermopsin is catalysed by CyrI, a 2-oxoglutarate-dependent iron oxygenase (2011), ChemBioChem, 12, 858-862 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 10702
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Kamptonema formosum PCC 6407
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Kamptonema sp. PCC 6506
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 7930
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus® (DE3)-RIL cells Oscillatoria sp. PCC 7926

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.70 7-deoxycylindrospermopsin substrate inhibition Oscillatoria sp. PCC 7926

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.70 0.0012
-
 7-deoxycylindrospermopsin pH and temperature not specified in the publication Oscillatoria sp. PCC 7926

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.70 Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 7926
1.14.11.70 Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 10702
1.14.11.70 Fe2+ dependent on, 0.05 mM used in assay conditions Kamptonema formosum PCC 6407
1.14.11.70 Fe2+ dependent on, 0.05 mM used in assay conditions Kamptonema sp. PCC 6506
1.14.11.70 Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 7930

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 10702
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema formosum PCC 6407
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema sp. PCC 6506
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7930
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 10702
-
 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema formosum PCC 6407
-
 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema sp. PCC 6506
-
 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7930
-
 cylindrospermopsin + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.70 Kamptonema formosum PCC 6407
-
-
-
1.14.11.70 Kamptonema sp. PCC 6506
-
-
-
1.14.11.70 Oscillatoria sp. PCC 10702
-
-
-
1.14.11.70 Oscillatoria sp. PCC 7926
-
-
-
1.14.11.70 Oscillatoria sp. PCC 7930
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.70 HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 7926
1.14.11.70 HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 10702
1.14.11.70 HisTrap Ni-affinity column chromatography Kamptonema formosum PCC 6407
1.14.11.70 HisTrap Ni-affinity column chromatography Kamptonema sp. PCC 6506
1.14.11.70 HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 7930

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 10702 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema formosum PCC 6407 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema sp. PCC 6506 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7930 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 10702 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema formosum PCC 6407 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema sp. PCC 6506 cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7930 cylindrospermopsin + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.70 ? x * 33581, quadrupole time-of-flight mass spectrometry Kamptonema sp. PCC 6506
1.14.11.70 ? x * 33582, calculated from amino acid sequence Kamptonema sp. PCC 6506
1.14.11.70 ? x * 33859, quadrupole time-of-flight mass spectrometry Oscillatoria sp. PCC 7926
1.14.11.70 ? x * 33860, calculated from amino acid sequence Oscillatoria sp. PCC 7926

Synonyms

EC Number Synonyms Comment Organism
1.14.11.70 cyrI
-
 Oscillatoria sp. PCC 7926
1.14.11.70 cyrI
-
 Oscillatoria sp. PCC 10702
1.14.11.70 cyrI
-
 Kamptonema formosum PCC 6407
1.14.11.70 cyrI
-
 Kamptonema sp. PCC 6506
1.14.11.70 cyrI
-
 Oscillatoria sp. PCC 7930

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.70 0.036
-
 7-deoxycylindrospermopsin pH and temperature not specified in the publication Oscillatoria sp. PCC 7926

General Information

EC Number General Information Comment Organism
1.14.11.70 metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 7926
1.14.11.70 metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 10702
1.14.11.70 metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Kamptonema formosum PCC 6407
1.14.11.70 metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Kamptonema sp. PCC 6506
1.14.11.70 metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 7930