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Literature summary extracted from

  • Baig, I.A.; Moon, J.Y.; Lee, S.C.; Ryoo, S.W.; Yoon, M.Y.
    Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase (2015), Biochim. Biophys. Acta, 1854, 1338-1350 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 additional information single stranded DNA aptamers Apt1 (CGAGTGAGGGCGAGGCGCGCTCCTGCCGGT) and Apt6 (CGGCCAGGGGACGAGCGCGCCCTGATCGTG) demonstrate the greatest inhibitory potential against the enzyme activity with IC50 values in the low nanomolar range (28.94 and 22.35 nM respectively). Aptamers Apt2, Apt3 and Apt4 show moderate to lower inhibition specificities Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ 10 mM used in assay conditions Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Mycobacterium tuberculosis
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate Mycobacterium tuberculosis H37Rv
-
 2-acetolactate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Mycobacterium tuberculosis
-
-
-
2.2.1.6 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 68000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Mycobacterium tuberculosis
2.2.1.6 AHAS
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate
-
 Mycobacterium tuberculosis