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Literature summary extracted from

  • Tramonti, A.; Nardella, C.; di Salvo, M.L.; Barile, A.; Cutruzzola, F.; Contestabile, R.
    Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison full kinetic characterization and substrate inhibition properties (2018), Biochemistry, 57, 6984-6996 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 L-serine
-
 Homo sapiens
2.1.2.1 tetrahydrofolate isoform SHMT2 maintains a pronounced tetrahydrofolate substrate inhibition even at the alkaline pH characteristic of the mitochondrial matrix Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.032
-
 tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
2.1.2.1 0.033
-
 tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 0.223
-
 tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 0.291
-
 5,10-methylenetetrahydrofolate isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.45
-
 L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
2.1.2.1 0.556
-
 glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.66
-
 glycine isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.98
-
 5,10-methylenetetrahydrofolate isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 2.2
-
 L-serine isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 4.8
-
 L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol isoform SHMT1 Homo sapiens 5829
-
2.1.2.1 mitochondrion isoform SHMT2 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Homo sapiens
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Homo sapiens P34896
-
-
2.1.2.1 Homo sapiens P34897
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Homo sapiens tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Homo sapiens
2.1.2.1 SHMT1 isoform Homo sapiens
2.1.2.1 SHMT2 isoform Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.188
-
 glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 6.23
-
 tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 13.55
-
 tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
2.1.2.1 14.17
-
 tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 8
-
-
 Homo sapiens
2.1.2.1 8.5
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.1 0.258
-
 tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 0.368
-
 L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 0.602
-
 L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 0.33
-
 glycine isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.52
-
 5,10-methylenetetrahydrofolate isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.65
-
 5,10-methylenetetrahydrofolate isoform SHMT1, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 0.77
-
 glycine isoform SHMT2, at pH 8.8 and 30°C Homo sapiens
2.1.2.1 1.33
-
 L-serine isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 6.5
-
 L-serine isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 30.17
-
 L-serine isoform SHMT2, at pH 7.8 and 30°C Homo sapiens
2.1.2.1 63.5
-
 tetrahydrofolate isoform SHMT2, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 188.83
-
 tetrahydrofolate isoform SHMT1, at pH 7.2 and 30°C Homo sapiens
2.1.2.1 423.33
-
 tetrahydrofolate isoform SHMT2, at pH 7.8 and 30°C Homo sapiens