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Literature summary extracted from

  • Hirashima, T.; Toyoshima, M.; Moriyama, T.; Nakamura, Y.; Sato, N.
    Characterization of phosphoethanolamine-N-methyltransferases in green algae (2017), Biochem. Biophys. Res. Commun., 488, 141-146 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 expressed in Escherichia coli Top10 cells Chlamydomonas applanata
2.1.1.103 expressed in Escherichia coli Top10 cells Chlamydomonas asymmetrica
2.1.1.103 expression Escherichia coli Chlamydomonas applanata
2.1.1.103 expression Escherichia coli Chlamydomonas asymmetrica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.0217
-
 Ethanolamine phosphate pH 8.0, 30°C Chlamydomonas applanata
2.1.1.103 0.0217
-
 Phosphoethanolamine at pH 8.0 and 30°C Chlamydomonas applanata
2.1.1.103 0.0292
-
 Ethanolamine phosphate pH 8.0, 30°C Chlamydomonas asymmetrica
2.1.1.103 0.0292
-
 Phosphoethanolamine at pH 8.0 and 30°C Chlamydomonas asymmetrica
2.1.1.103 0.0639
-
 S-adenosyl-L-methionine pH 8.0, 30°C Chlamydomonas applanata
2.1.1.103 0.0639
-
 S-adenosyl-L-methionine at pH 8.0 and 30°C Chlamydomonas applanata
2.1.1.103 0.106
-
 S-adenosyl-L-methionine pH 8.0, 30°C Chlamydomonas asymmetrica
2.1.1.103 0.106
-
 S-adenosyl-L-methionine at pH 8.0 and 30°C Chlamydomonas asymmetrica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Chlamydomonas applanata A0A1W7HP20
-
-
2.1.1.103 Chlamydomonas applanata NIES-2202 A0A1W7HP20
-
-
2.1.1.103 Chlamydomonas asymmetrica A0A125SQH6
-
-
2.1.1.103 Chlamydomonas asymmetrica NIES-2207 A0A125SQH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 HisTrap column chromatography Chlamydomonas applanata
2.1.1.103 HisTrap column chromatography Chlamydomonas asymmetrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 additional information no substrate: phosphomonomethylethanolamine Chlamydomonas applanata ?
-
-
2.1.1.103 additional information no substrate: phosphomonomethylethanolamine Chlamydomonas asymmetrica ?
-
-
2.1.1.103 additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas applanata ?
-
-
2.1.1.103 additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas asymmetrica ?
-
-
2.1.1.103 additional information no substrate: phosphomonomethylethanolamine Chlamydomonas asymmetrica NIES-2207 ?
-
-
2.1.1.103 additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas asymmetrica NIES-2207 ?
-
-
2.1.1.103 additional information no substrate: phosphomonomethylethanolamine Chlamydomonas applanata NIES-2202 ?
-
-
2.1.1.103 additional information the enzyme shows no methyltransferase activity with phosphomonomethylethanolamine Chlamydomonas applanata NIES-2202 ?
-
-
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas applanata S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas asymmetrica S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas asymmetrica NIES-2207 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Chlamydomonas applanata NIES-2202 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas applanata S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas asymmetrica S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas asymmetrica NIES-2207 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine
-
 Chlamydomonas applanata NIES-2202 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.103 ? x * 67900, calculated from amino acid sequence Chlamydomonas applanata
2.1.1.103 ? x * 69700, SDS-PAGE, thioredoxin-tagged recombinant protein Chlamydomonas applanata
2.1.1.103 ? x * 75300, calculated from amino acid sequence Chlamydomonas asymmetrica
2.1.1.103 ? x * 75300, SDS-PAGE, thioredoxin-tagged recombinant protein Chlamydomonas asymmetrica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.103 PEAMT
-
 Chlamydomonas applanata
2.1.1.103 PEAMT
-
 Chlamydomonas asymmetrica
2.1.1.103 phosphoethanolamine-N-methyltransferases
-
 Chlamydomonas applanata
2.1.1.103 phosphoethanolamine-N-methyltransferases
-
 Chlamydomonas asymmetrica