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Literature summary extracted from

  • VanDrisse, C.M.; Hentchel, K.L.; Escalante-Semerena, J.C.
    Phosphinothricin acetyltransferases identified using in vivo, in vitro, and bioinformatic analyses (2016), Appl. Environ. Microbiol., 82, 7041-7051 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.183 expression in Escherichia coli C41(lambdaDE3) Geobacillus kaustophilus
2.3.1.183 expression in Escherichia coli C41(lambdaDE3) Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.183 M55stop the active site of the mutant enzyme remains functional but is sufficiently altered to prevent acetylation of phosphinothricin Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.183 0.004
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 0.005
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 0.017
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 0.089
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 0.121
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 0.198
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 0.316
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 0.721
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.183 Deinococcus radiodurans Q9RV48
-
-
2.3.1.183 Deinococcus radiodurans ATCC 13939 Q9RV48
-
-
2.3.1.183 Geobacillus kaustophilus Q5KVT1
-
-
2.3.1.183 Geobacillus kaustophilus Q5L2F2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.183
-
 Geobacillus kaustophilus
2.3.1.183
-
 Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.183 acetyl-CoA + L-methionine sulfone
-
 Geobacillus kaustophilus CoA + N-acetyl-L-methionine sulfone
-
-
2.3.1.183 acetyl-CoA + L-methionine sulfone
-
 Deinococcus radiodurans CoA + N-acetyl-L-methionine sulfone
-
-
2.3.1.183 acetyl-CoA + L-methionine sulfone
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetyl-L-methionine sulfone
-
-
2.3.1.183 acetyl-CoA + L-methionine sulfoximine
-
 Geobacillus kaustophilus CoA + N-acetyl-L-methionine sulfoxime
-
-
2.3.1.183 acetyl-CoA + L-methionine sulfoximine
-
 Deinococcus radiodurans CoA + N-acetyl-L-methionine sulfoxime
-
-
2.3.1.183 acetyl-CoA + L-methionine sulfoximine
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetyl-L-methionine sulfoxime
-
-
2.3.1.183 acetyl-CoA + phosphinothricin
-
 Geobacillus kaustophilus CoA + N-acetylphosphinothricin
-
 ?
2.3.1.183 acetyl-CoA + phosphinothricin
-
 Deinococcus radiodurans CoA + N-acetylphosphinothricin
-
 ?
2.3.1.183 acetyl-CoA + phosphinothricin the active site of the mutant enzyme remains functional but is sufficiently altered to prevent acetylation of phosphinothricin Geobacillus kaustophilus CoA + N-acetylphosphinothricin
-
 ?
2.3.1.183 acetyl-CoA + phosphinothricin
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetylphosphinothricin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.183 DR_1182
-
 Deinococcus radiodurans
2.3.1.183 GK0593
-
 Geobacillus kaustophilus
2.3.1.183 GK2920
-
 Geobacillus kaustophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.183 1
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 1
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 1
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 5
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 9
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 10
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 11
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 11
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.183 7
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 30
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 58.8
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 60
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
2.3.1.183 74.3
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 112.4
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
2.3.1.183 200
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
2.3.1.183 250
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus