BRENDA - Enzyme Database

Structural and functional survey of environmental aminoglycoside acetyltransferases reveals functionality of resistance enzymes

Xu, Z.; Stogios, P.J.; Quaile, A.T.; Forsberg, K.J.; Patel, S.; Skarina, T.; Houliston, S.; Arrowsmith, C.; Dantas, G.; Savchenko, A.; ACS Infect. Dis. 3, 653-665 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.60
expressed in Escherichia coli BL21(DE3)-Gold cells
uncultured bacterium
2.3.1.60
expressed in Escherichia coli BL21(DE3)-Gold cells
Pseudomonas aeruginosa
2.3.1.82
expressed in Escherichia coli BL21(DE3)-Gold cells
uncultured bacterium
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.3.1.60
apoenzyme, sitting drop vapor diffusion method, using 40% (w/v) PEG 300 and phosphate-citrate (pH 5.2)
Pseudomonas aeruginosa
2.3.1.60
in complex with CoA, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M magnesium formate
uncultured bacterium
2.3.1.82
in complex with sisomicin
uncultured bacterium
2.3.1.82
meta-AAC0020 enzyme mutant Y138A in complex with sisomicin, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 0.2 M calcium acetate. The apoenzyme is crystallized in a solution containing 4 M sodium formate
uncultured bacterium
Engineering
EC Number
Protein Variants
Commentary
Organism
2.3.1.82
Y138A
catalytically inactive
uncultured bacterium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.021
-
acetyl-CoA
K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.022
-
gentamycin
K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.028
-
tobramycin
K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.038
-
sisomicin
K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.346
-
kanamycin
K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C
uncultured bacterium
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.60
Pseudomonas aeruginosa
Q51407
-
-
2.3.1.60
uncultured bacterium
-
-
-
2.3.1.60
uncultured bacterium
A0A059WV44
-
-
2.3.1.82
uncultured bacterium
A0A059WZ16
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.60
Ni-NTA column chromatography and Superdex S200 gel filtration
uncultured bacterium
2.3.1.60
Ni-NTA column chromatography and Superdex S200 gel filtration
Pseudomonas aeruginosa
2.3.1.82
Ni-NTA column chromatography and Superdex S200 gel filtration
uncultured bacterium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.60
acetyl-CoA + gentamicin
-
755693
uncultured bacterium
CoA + N3-acetylgentamicin
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylgentamicin
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin
-
755693
uncultured bacterium
CoA + N3-acetylkanamycin
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylkanamycin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + N3-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + N3-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamycin
-
755693
uncultured bacterium
CoA + 6'-N-acetylgentamycin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin
-
755693
uncultured bacterium
CoA + 6'-N-acetylkanamycin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
755693
uncultured bacterium
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin C
-
755693
uncultured bacterium
CoA + N6'-acetylkanamycin C
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + N6'-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + 6'-N-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + 6'-N-acetyltobramycin
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.60
AAC
-
uncultured bacterium
2.3.1.60
AAC
-
Pseudomonas aeruginosa
2.3.1.60
AAC(3)-Ib
-
Pseudomonas aeruginosa
2.3.1.60
aminoglycoside acetyltransferase
-
uncultured bacterium
2.3.1.60
aminoglycoside acetyltransferase
-
Pseudomonas aeruginosa
2.3.1.60
aminoglycoside N-acetyltransferase
-
uncultured bacterium
2.3.1.60
aminoglycoside N-acetyltransferase
-
Pseudomonas aeruginosa
2.3.1.60
meta-AAC0005
-
uncultured bacterium
2.3.1.60
meta-AAC0007
-
uncultured bacterium
2.3.1.60
meta-AAC0012
-
uncultured bacterium
2.3.1.82
AAC
-
uncultured bacterium
2.3.1.82
AAC(6')
-
uncultured bacterium
2.3.1.82
AAC3-I
-
uncultured bacterium
2.3.1.82
aminoglycoside acetyltransferase
-
uncultured bacterium
2.3.1.82
aminoglycoside N-acetyltransferase
-
uncultured bacterium
2.3.1.82
meta-AAC0020
-
uncultured bacterium
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.207
-
kanamycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.17
-
gentamycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.2
-
tobramycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.51
-
acetyl-CoA
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
2.42
-
sisomicin
pH 7.5, 25°C
uncultured bacterium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.60
expressed in Escherichia coli BL21(DE3)-Gold cells
uncultured bacterium
2.3.1.60
expressed in Escherichia coli BL21(DE3)-Gold cells
Pseudomonas aeruginosa
2.3.1.82
expressed in Escherichia coli BL21(DE3)-Gold cells
uncultured bacterium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.3.1.60
apoenzyme, sitting drop vapor diffusion method, using 40% (w/v) PEG 300 and phosphate-citrate (pH 5.2)
Pseudomonas aeruginosa
2.3.1.60
in complex with CoA, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M magnesium formate
uncultured bacterium
2.3.1.82
in complex with sisomicin
uncultured bacterium
2.3.1.82
meta-AAC0020 enzyme mutant Y138A in complex with sisomicin, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 0.2 M calcium acetate. The apoenzyme is crystallized in a solution containing 4 M sodium formate
uncultured bacterium
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.3.1.82
Y138A
catalytically inactive
uncultured bacterium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.021
-
acetyl-CoA
K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.022
-
gentamycin
K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.028
-
tobramycin
K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.038
-
sisomicin
K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
0.346
-
kanamycin
K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C
uncultured bacterium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.60
Ni-NTA column chromatography and Superdex S200 gel filtration
uncultured bacterium
2.3.1.60
Ni-NTA column chromatography and Superdex S200 gel filtration
Pseudomonas aeruginosa
2.3.1.82
Ni-NTA column chromatography and Superdex S200 gel filtration
uncultured bacterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.60
acetyl-CoA + gentamicin
-
755693
uncultured bacterium
CoA + N3-acetylgentamicin
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylgentamicin
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin
-
755693
uncultured bacterium
CoA + N3-acetylkanamycin
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylkanamycin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + N3-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + N3-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
755693
Pseudomonas aeruginosa
CoA + N3-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamycin
-
755693
uncultured bacterium
CoA + 6'-N-acetylgentamycin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin
-
755693
uncultured bacterium
CoA + 6'-N-acetylkanamycin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
755693
uncultured bacterium
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin C
-
755693
uncultured bacterium
CoA + N6'-acetylkanamycin C
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + N6'-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
755693
uncultured bacterium
CoA + 6'-N-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
755693
uncultured bacterium
CoA + 6'-N-acetyltobramycin
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.207
-
kanamycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.17
-
gentamycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.2
-
tobramycin
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
1.51
-
acetyl-CoA
pH 7.5, 25°C
uncultured bacterium
2.3.1.82
2.42
-
sisomicin
pH 7.5, 25°C
uncultured bacterium
General Information
EC Number
General Information
Commentary
Organism
2.3.1.60
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
uncultured bacterium
2.3.1.60
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
Pseudomonas aeruginosa
2.3.1.82
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
uncultured bacterium
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.60
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
uncultured bacterium
2.3.1.60
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
Pseudomonas aeruginosa
2.3.1.82
physiological function
the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin
uncultured bacterium
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.6
-
kanamycin
K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
43
-
tobramycin
K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
52
-
gentamycin
K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
64
-
sisomicin
K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
69
-
acetyl-CoA
K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C
uncultured bacterium
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.6
-
kanamycin
K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
43
-
tobramycin
K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
52
-
gentamycin
K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
64
-
sisomicin
K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C
uncultured bacterium
2.3.1.82
69
-
acetyl-CoA
K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C
uncultured bacterium