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Literature summary extracted from

  • Stogios, P.; Kuhn, M.; Evdokimova, E.; Law, M.; Courvalin, P.; Savchenko, A.
    Structural and biochemical characterization of Acinetobacter spp. aminoglycoside acetyltransferases highlights functional and evolutionary variation among antibiotic resistance enzymes (2017), ACS Infect. Dis., 3, 132-143 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.82 structure of AAC(6')-Ig in complex with tobramycin reveals a large substrate-binding cleft remaining partially unoccupied by the substrate Acinetobacter haemolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.0029
-
 tobramicin S 0.5 value, Hill coefficient 2.12, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0059
-
 arbekacin S 0.5 value, Hill coefficient 2.5, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0062
-
 ribostamycin S 0.5 value, Hill coefficient 1.92, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0074
-
 neomycin C S 0.5 value, Hill coefficient 1.43, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0079
-
 neomycin C S 0.5 value, Hill coefficient 2.05, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0087
-
 gentamicin S 0.5 value, Hill coefficient 1.84, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0098
-
 dibekacin S 0.5 value, Hill coefficient 1.42, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0105
-
 sisomicin S 0.5 value, Hill coefficient 1.93, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0118
-
 ribostamycin S 0.5 value, Hill coefficient 1.96, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.012
-
 kanamycin B S 0.5 value, Hill coefficient 2.30, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0139
-
 arbekacin S 0.5 value, Hill coefficient 2.19, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0168
-
 sisomicin S 0.5 value, Hill coefficient 2.04, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0173
-
 butirosin A S 0.5 value, Hill coefficient 1.77, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0176
-
 gentamicin S 0.5 value, Hill coefficient 2.05, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0183
-
 2'-N-ethylnetilmicin S 0.5 value, Hill coefficient 2.28, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0216
-
 butirosin A S 0.5 value, Hill coefficient 2.03, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.022
-
 tobramicin S 0.5 value, Hill coefficient 1.21, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0259
-
 dibekacin S 0.5 value, Hill coefficient 1.26, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.029
-
 kanamycin B S 0.5 value, Hill coefficient 1.93, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.0441
-
 amikacin S 0.5 value, Hill coefficient 1.55, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.049
-
 2'-N-ethyl netilmicin S 0.5 value, Hill coefficient 1.39, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.0996
-
 amikacin S 0.5 value, Hill coefficient 1.26, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.104
-
 isepamicin S 0.5 value, Hill coefficient 1.45, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.111
-
 isepamicin S 0.5 value, Hill coefficient 1.48, pH 8.0, 37°C Acinetobacter baumannii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Acinetobacter baumannii Q43899
-
-
2.3.1.82 Acinetobacter haemolyticus Q44057
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + 2'-N-ethyl netilmicin
-
 Acinetobacter baumannii CoA + N6'-acetyl-2'-N-ethylnetilmicin
-
 ?
2.3.1.82 acetyl-CoA + 2'-N-ethylnetilmicin
-
 Acinetobacter haemolyticus CoA + N6'-acetyl-2'-N-ethylnetilmicin
-
 ?
2.3.1.82 acetyl-CoA + amikacin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + amikacin
-
 Acinetobacter baumannii CoA + 6'-N-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + arbekacin
-
 Acinetobacter haemolyticus CoA + N6'-acetylarbekacin
-
 ?
2.3.1.82 acetyl-CoA + arbekacin
-
 Acinetobacter baumannii CoA + N6'-acetylarbekacin
-
 ?
2.3.1.82 acetyl-CoA + butirosin A
-
 Acinetobacter haemolyticus CoA + 6'-N-acetyl-butirosin A
-
 ?
2.3.1.82 acetyl-CoA + butirosin A
-
 Acinetobacter baumannii CoA + 6'-N-acetyl-butirosin A
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Acinetobacter baumannii CoA + 6'-N-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylgentamicin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin
-
 Acinetobacter baumannii CoA + 6'-N-acetylgentamicin
-
 ?
2.3.1.82 acetyl-CoA + isepamicin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylisepamicin
-
 ?
2.3.1.82 acetyl-CoA + isepamicin
-
 Acinetobacter baumannii CoA + 6'-N-acetylisepamicin
-
 ?
2.3.1.82 acetyl-CoA + kanamycin B
-
 Acinetobacter haemolyticus CoA + N6'-acetylkanamycin B
-
 ?
2.3.1.82 acetyl-CoA + kanamycin B
-
 Acinetobacter baumannii CoA + N6'-acetylkanamycin B
-
 ?
2.3.1.82 acetyl-CoA + neomycin C
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylneomycin C
-
 ?
2.3.1.82 acetyl-CoA + neomycin C
-
 Acinetobacter baumannii CoA + 6'-N-acetylneomycin C
-
 ?
2.3.1.82 acetyl-CoA + ribostamycin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylribostamycin
-
 ?
2.3.1.82 acetyl-CoA + ribostamycin
-
 Acinetobacter baumannii CoA + 6'-N-acetylribostamycin
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 Acinetobacter baumannii CoA + 6'-N-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + tobramicin
-
 Acinetobacter haemolyticus CoA + 6'-N-acetyltobramicin
-
 ?
2.3.1.82 acetyl-CoA + tobramicin
-
 Acinetobacter baumannii CoA + 6'-N-acetyltobramicin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(6')-Ig
-
 Acinetobacter haemolyticus
2.3.1.82 AAC(6')-Ih
-
 Acinetobacter baumannii
2.3.1.82 aminoglycoside N(6')-acetyltransferase type 1
-
 Acinetobacter haemolyticus
2.3.1.82 aminoglycoside N(6')-acetyltransferase type 1
-
 Acinetobacter baumannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.016
-
 gentamicin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.021
-
 arbekacin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.022
-
 tobramicin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.04
-
 ribostamycin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.04
-
 dibekacin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.051
-
 sisomicin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.055
-
 gentamicin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.057
-
 neomycin C pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.06
-
 kanamycin B pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.06
-
 neomycin C pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.072
-
 ribostamycin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.077
-
 arbekacin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.085
-
 tobramicin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.097
-
 2'-N-ethylnetilmicin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.098
-
 2'-N-ethyl netilmicin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.107
-
 butirosin A pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.113
-
 butirosin A pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.127
-
 dibekacin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.13
-
 sisomicin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.181
-
 kanamycin B pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.328
-
 amikacin pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 0.496
-
 amikacin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.62
-
 isepamicin pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 0.779
-
 isepamicin pH 8.0, 37°C Acinetobacter haemolyticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.82 1.84
-
 gentamicin kcat/S 0.5 value, Hill coefficient 1.84, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 2
-
 2'-N-ethyl netilmicin kcat/S 0.5 value, Hill coefficient 1.39, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 3.13
-
 gentamicin kcat/S 0.5 valuevalue, Hill coefficient 2.05, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 3.56
-
 arbekacin kcat/S 0.5 value, Hill coefficient 2.5, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 3.86
-
 tobramicin kcat/S 0.5 valuevalue, Hill coefficient 1.21, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 4.08
-
 dibekacin kcat/S 0.5 value, Hill coefficient 1.42, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 4.86
-
 sisomicin kcat/S 0.5 value, Hill coefficient 1.93, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 4.9
-
 dibekacin kcat/S 0.5 valuevalue, Hill coefficient 1.26, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 4.98
-
 amikacin kcat/S 0.5 value, Hill coefficient 1.26, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 5
-
 kanamycin B kcat/S 0.5 value, Hill coefficient 2.30, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 5.23
-
 butirosin A kcat/S 0.5 value, Hill coefficient 2.03, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 5.3
-
 2'-N-ethylnetilmicin kcat/S 0.5 valuevalue, Hill coefficient 2.28, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 5.54
-
 arbekacin kcat/S 0.5 valuevalue, Hill coefficient 2.19, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 5.59
-
 isepamicin kcat/S 0.5 value, Hill coefficient 1.48, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 6.1
-
 ribostamycin kcat/S 0.5 value, Hill coefficient 1.96, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 6.18
-
 butirosin A kcat/S 0.5 valuevalue, Hill coefficient 1.77, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 6.24
-
 kanamycin B kcat/S 0.5 valuevalue, Hill coefficient 1.93, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 7.1
-
 ribostamycin kcat/S 0.5 value, Hill coefficient 1.92, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 7.44
-
 amikacin kcat/S 0.5 value, Hill coefficient 1.55, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 7.49
-
 isepamicin kcat/S 0.5 valuevalue, Hill coefficient 1.45, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 7.59
-
 neomycin C kcat/S 0.5 value, Hill coefficient 2.05, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 7.59
-
 tobramicin kcat/S 0.5 value, Hill coefficient 2.12, pH 8.0, 37°C Acinetobacter baumannii
2.3.1.82 7.7
-
 neomycin C kcat/S 0.5 value, Hill coefficient 1.43, pH 8.0, 37°C Acinetobacter haemolyticus
2.3.1.82 7.74
-
 sisomicin kcat/S 0.5 valuevalue, Hill coefficient 2.04, pH 8.0, 37°C Acinetobacter haemolyticus