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Literature summary extracted from

  • Nguyen, H.A.; Durden, D.L.; Lavie, A.
    The differential ability of asparagine and glutamine in promoting the closed/active enzyme conformation rationalizes the Wolinella succinogenes L-asparaginase substrate specificity (2017), Sci. Rep., 7, 41643 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 wild-type and mutant S121P. Residue 121 impacts the conformation of the conserved tyrosine 27, a component of the catalytically-important flexible N-terminal loop Wolinella succinogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 S121P mutant gains L-glutaminase activity, but retains L-asparaginase activity comparable to wild-type Wolinella succinogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.0217
-
L-asparagine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 0.0236
-
L-asparagine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 0.32
-
L-glutamine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 0.38
-
L-glutamine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Wolinella succinogenes P50286
-
-
3.5.1.1 Wolinella succinogenes DSM 1740 P50286
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
Wolinella succinogenes L-aspartate + NH3
-
?
3.5.1.1 L-asparagine + H2O
-
Wolinella succinogenes DSM 1740 L-aspartate + NH3
-
?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Wolinella succinogenes L-glutamate + NH3
-
?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Wolinella succinogenes DSM 1740 L-glutamate + NH3
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 1.58
-
L-glutamine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 11.33
-
L-glutamine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 86.7
-
L-asparagine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 97.8
-
L-asparagine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 4.2
-
L-glutamine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 35.3
-
L-glutamine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 3700
-
L-asparagine mutant S121P, 37°C, pH not specified in the publication Wolinella succinogenes
3.5.1.1 4500
-
L-asparagine wild-type, 37°C, pH not specified in the publication Wolinella succinogenes