EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.1 | wild-type and mutant S121P. Residue 121 impacts the conformation of the conserved tyrosine 27, a component of the catalytically-important flexible N-terminal loop | Wolinella succinogenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.1 | S121P | mutant gains L-glutaminase activity, but retains L-asparaginase activity comparable to wild-type | Wolinella succinogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.0217 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 0.0236 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 0.32 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 0.38 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Wolinella succinogenes | P50286 | - |
- |
3.5.1.1 | Wolinella succinogenes DSM 1740 | P50286 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Wolinella succinogenes | L-aspartate + NH3 | - |
? | |
3.5.1.1 | L-asparagine + H2O | - |
Wolinella succinogenes DSM 1740 | L-aspartate + NH3 | - |
? | |
3.5.1.1 | L-glutamine + H2O | reaction of EC 3.5.1.2 | Wolinella succinogenes | L-glutamate + NH3 | - |
? | |
3.5.1.1 | L-glutamine + H2O | reaction of EC 3.5.1.2 | Wolinella succinogenes DSM 1740 | L-glutamate + NH3 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 1.58 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 11.33 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 86.7 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 97.8 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 4.2 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 35.3 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 3700 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3.5.1.1 | 4500 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |