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Literature summary extracted from
- A protease-resistant Escherichia coli asparaginase with outstanding stability and enhanced anti-leukaemic activity in vitro (2017), Sci. Rep., 7, 14479 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | - |
Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | N24S | mutant shows completely preserved asparaginase and glutaminase activities, long-term storage stability, improved thermal parameters, and good resistance to proteases derived from leukaemia cells. The mutant displays a modification in the hydrogen bond network related to residue 24, and a general rigidification of the monomer as compared to wild-type | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 0.03 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |  |
| 3.5.1.1 | 0.03 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 3.95 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 4.14 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Escherichia coli | P00805 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 103 | - |
mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
| 3.5.1.1 | 105 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | L-glutamine + H2O | reaction of EC 3.5.1.2 | Escherichia coli | L-glutamate + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | AnsB | - |
Escherichia coli |
| 3.5.1.1 | L-asparaginase 2 | - |
Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 49 | - |
melting temperature, wild-type | Escherichia coli |
| 3.5.1.1 | 59 | - |
melting temperature, mutant N24S | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 0.51 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 0.53 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 58.8 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 59.8 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 0.13 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 0.13 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 1782 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5.1.1 | 1975 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
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