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Literature summary extracted from

  • Costa, I.M.; Schultz, L.; de Araujo Bianchi Pedra, B.; Leite, M.S.; Farsky, S.H.; de Oliveira, M.A.; Pessoa, A.; Monteiro, G.
    Recombinant L-asparaginase 1 from Saccharomyces cerevisiae an allosteric enzyme with antineoplastic activity (2016), Sci. Rep., 6, 36239 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.1 medicine the purified enzyme shows cytotoxicity for the MOLT-4 leukemic cell lineage Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 K215A 99.9% loss of activity Saccharomyces cerevisiae
3.5.1.1 additional information residues T64, Y78, T141, K15 are involved in catalysis Saccharomyces cerevisiae
3.5.1.1 T141A 99.9% loss of activity Saccharomyces cerevisiae
3.5.1.1 T64A 99.9% loss of activity Saccharomyces cerevisiae
3.5.1.1 Y78A 99.9% loss of activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.075
-
 L-asparagine K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae
3.5.1.1 0.122
-
 L-asparagine presence of L-glutamine, K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Saccharomyces cerevisiae
-
-
-
3.5.1.1 Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant protein Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 196.2
-
 pH 8.8, 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Saccharomyces cerevisiae L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Saccharomyces cerevisiae BY4741 L-aspartate + NH3
-
 ?
3.5.1.1 additional information very poor substrate: L-glutamine Saccharomyces cerevisiae ?
-
 ?
3.5.1.1 additional information very poor substrate: L-glutamine Saccharomyces cerevisiae BY4741 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 41400, calculated from sequence, x * 45000, His-tagged recombinant protein, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASNase1
-
 Saccharomyces cerevisiae
3.5.1.1 ASP1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 40
-
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 217
-
 L-asparagine K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae
3.5.1.1 523
-
 L-asparagine presence of L-glutamine, K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8.6
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 5 11
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 13
-
 L-asparagine presence of L-glutamine, K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae
3.5.1.1 16
-
 L-asparagine K0.5 value, pH 8.8, 37°C Saccharomyces cerevisiae