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Literature summary extracted from

  • Saeed, H.; Soudan, H.; El-Sharkawy, A.; Farag, A.; Embaby, A.; Ataya, F.
    Expression and functional characterization of Pseudomonas aeruginosa recombinant L.asparaginase (2018), Protein J., 37, 461-471 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine purified L-asparaginase does not show hemolysis effect on blood erythrocytes. Recombinant L-asparaginase retains 50% of its initial activity after 90 and 60 min incubation in serum and trypsin separately Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1
-
 Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 molecular modeling of structure Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Fe2+ 5 mM, 82% of initial activity Pseudomonas aeruginosa
3.5.1.1 Zn2+ 5 mM, 72% of initial activity Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ 5 mM, 122% of initial activity Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 72400
-
 PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant protein Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 5925
-
 pH not specified in the publication, temperature not specified in the publication Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 acrylamide + H2O
-
 Pseudomonas aeruginosa acrylic acid + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas aeruginosa L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Pseudomonas aeruginosa L-glutamate + NH3
-
 ?
3.5.1.1 urea + H2O
-
 Pseudomonas aeruginosa ? + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer 2 * 36300, SDS-PAGE, 2 * 36276, calculated from sequence Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Pseudomonas aeruginosa calculated from sequence
-
 4.7