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Literature summary extracted from

  • Kee, N.L.; Krause, J.; Blatch, G.L.; Muramoto, K.; Sakka, K.; Sakka, M.; Naude, R.J.; Wagner, L.; Wolf, R.; Rahfeld, J.U.; Demuth, H.U.; Mielicki, W.P.; Frost, C.L.
    The proteolytic profile of human cancer procoagulant suggests that it promotes cancer metastasis at the level of activation rather than degradation (2015), Protein J., 34, 338-348 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.26 (NH4)2SO4 76% inhibition at about 40 mM Homo sapiens
3.4.22.26 benzamidine strong inhibition Homo sapiens
3.4.22.26 CaCl2 91% inhibition at about 40 mM Homo sapiens
3.4.22.26 EDTA low inhibition Homo sapiens
3.4.22.26 HgCl2 complete inhibition at about 40 mM Homo sapiens
3.4.22.26 MgCl2 81% inhibition at about 40 mM Homo sapiens
3.4.22.26 MgSO4 68% inhibition at about 40 mM Homo sapiens
3.4.22.26 additional information no or poor inhibition by iodoacetamide and E-64 Homo sapiens
3.4.22.26 NaCl 12-21% inhibition, respectively Homo sapiens
3.4.22.26 pepstatin A low inhibition Homo sapiens
3.4.22.26 Phenylmethylsulfonylfluoride strong inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.26 0.01182
-
 tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 0.2265
-
 PQVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 0.2675
-
 QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 0.3463
-
 QSPVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 0.3933
-
 LPAPR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.26 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.26 inactive factor X + H2O Homo sapiens activation of blood coagulation factor X, degradation of factor X during 24 h active factor Xa + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.26 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.26 native enzyme from human placental membranes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.26 additional information tumor cell Homo sapiens
-
3.4.22.26 placental membrane
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.26 7-methoxycoumarin-4-acetyl-GYPGQV + H2O
-
 Homo sapiens 7-methoxycoumarin-4-acetate + GYPGQV
-
 ?
3.4.22.26 7-methoxycoumarin-4-acetyl-IVGGQE + H2O
-
 Homo sapiens 7-methoxycoumarin-4-acetate + IVGGQE
-
 ?
3.4.22.26 7-methoxycoumarin-4-acetyl-SFLLRN + H2O
-
 Homo sapiens 7-methoxycoumarin-4-acetate + SFLLRN
-
 ?
3.4.22.26 7-methoxycoumarin-4-acetyl-VSQKSP + H2O
-
 Homo sapiens 7-methoxycoumarin-4-acetate + VSQKSP
-
 ?
3.4.22.26 7-methoxycoumarin-4-acetyl-VYQPQP + H2O
-
 Homo sapiens 7-methoxycoumarin-4-acetate + VYQPQP
-
 ?
3.4.22.26 collagen type IV + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.26 Fibronectin + H2O enzyme CP cleaves fibronectin between Ala and Val residues, in regions rich in Pro and Gln residues. The 45 and 60 kDa fragments have the same N-terminal sequence, generated by cleavage of the bond between Ala292 and Val293 Homo sapiens ?
-
 ?
3.4.22.26 inactive factor X + H2O activation of blood coagulation factor X, degradation of factor X during 24 h Homo sapiens active factor Xa + ?
-
 ?
3.4.22.26 inactive factor X + H2O activation of blood coagulation factor X (57.3 kDa), cleavage of factor X at the conventional activation site, i.e. between Arg52 and Ile53, in the heavy chain Homo sapiens active factor Xa + ?
-
 ?
3.4.22.26 Laminin + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.26 LPAPR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens LPAPR + 7-amino-4-methylcoumarin
-
 ?
3.4.22.26 additional information substrate specificity analysis, mass spectrometric detection of cleavage products. No activity with tert-benzyloxycarbonyl-QSPVR-7-amido-4-methylcoumarin and TLDPR-7-amido-4-methylcoumarin. Enzyme CP shows very little cysteine protease activity, it appears to exhibit mixed protease activity, but acts predominantly as a serine protease Homo sapiens ?
-
 ?
3.4.22.26 PQVR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens PQVR + 7-amino-4-methylcoumarin
-
 ?
3.4.22.26 QSPVR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens QSPVR + 7-amino-4-methylcoumarin
-
 ?
3.4.22.26 QVR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens QVR + 7-amino-4-methylcoumarin
-
 ?
3.4.22.26 tert-butoxycarbonyl-QVR-7-amido-4-methylcoumarin + H2O best synthetic substrate Homo sapiens tert-butoxycarbonyl-QVR + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.26 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.26 0.989
-
 tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 1.147
-
 QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 2.496
-
 QSPVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 3.942
-
 LPAPR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 4.578
-
 PQVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.26 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.22.26 physiological function cancer procoagulant (CP) is a protease present in malignant tumours, but not normal tissue, is a known activator of coagulation factor X (FX). degradation of FX by CP occurs at a much slower rate than degradation by conventional activators. Cancer procoagulant has a function in cancer progression. The enzyme is not essential for either cancer-associated blood coagulation or the degradation of extracellular matrix proteins. The protease may be required for the proteolytic activation of membrane receptors Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.26 4.288
-
 QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 7.208
-
 QSPVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 10.02
-
 LPAPR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 20.21
-
 PQVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
3.4.22.26 83.67
-
 tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens