Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • More, S.; Swamy, R.; Mohan, N.; Navyashree, M.; Janardhan, B.; Niyonzima, F.
    Purification and characterization of anti-cancer L-glutaminase of Bacillus cereus strain LC13 (2018), Proc. Natl. Acad. Sci. India: Sect. B Biol. Sci., 88, 695-705 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.2 synthesis optimal conditions for native enzyme production are pH 7.0,37°C and 0.3 % L-glutamine. Maltose (0.4 %, w/v) is best carbon source supplement Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 Co2+ 5 mM, 81% of initial activity Bacillus cereus
3.5.1.2 Cu2+ 5 mM, 86% of initial activity Bacillus cereus
3.5.1.2 dithiothreitol 5 mM, 82% of initial activity Bacillus cereus
3.5.1.2 Fe2+ 5 mM, 83% of initial activity Bacillus cereus
3.5.1.2 Fe3+ 5 mM, 84% of initial activity Bacillus cereus
3.5.1.2 Hg2+ 5 mM, 61% of initial activity Bacillus cereus
3.5.1.2 iodoacetamide 5 mM, 3% of initial activity Bacillus cereus
3.5.1.2 additional information phenylmethylsulfonyl fluoride, sodium azide, ethylenediaminetetraacetic acid and N-acetylimidazole have no effect on enzyme Bacillus cereus
3.5.1.2 N-bromosuccinimide 5 mM, 19% of initial activity Bacillus cereus
3.5.1.2 N-ethylmaleimide 5 mM, 8% of initial activity Bacillus cereus
3.5.1.2 TLCK 5 mM, 12% of initial activity Bacillus cereus
3.5.1.2 Zn2+ 5 mM, 79% of initial activity Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.2 soluble
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 K+ 5 mM, 113% of initial activity Bacillus cereus
3.5.1.2 Na+ 5 mM, 114% of initial activity Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Bacillus cereus
-
-
-
3.5.1.2 Bacillus cereus LC13
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 58.6
-
 pH 7.0, 37°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 L-glutamine + H2O
-
 Bacillus cereus L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Bacillus cereus LC13 L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 ? x * 35000, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 30 40
-
 Bacillus cereus