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Literature summary extracted from

  • Dash, C.; Mohapatra, S.; Maiti, P.
    Optimization, purification, and characterization of L-asparaginase from Actinomycetales bacterium BkSoiiA (2016), Prep. Biochem. Biotechnol., 46, 1-7 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 dithiothreitol 10 mM, 116% of initial activity Actinomycetales bacterium

Application

EC Number Application Comment Organism
3.5.1.1 synthesis production and optimization of growth conditions results in submerged fermentation in modified M9 medium with yeast extract and fructose as carbon and nitrogen sources, respectively, at pH 8.0, incubated for 120 h at 30°C Actinomycetales bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Ba2+ 10 mM, 12% residual activity Actinomycetales bacterium
3.5.1.1 Ca2+ 10 mM, 28% residual activity Actinomycetales bacterium
3.5.1.1 Cu2+ 10 mM, 23% residual activity Actinomycetales bacterium
3.5.1.1 Fe2+ 10 mM, 44% residual activity Actinomycetales bacterium
3.5.1.1 Hg2+ 10 mM, 33% residual activity Actinomycetales bacterium
3.5.1.1 Li+ 10 mM, 17% residual activity Actinomycetales bacterium
3.5.1.1 additional information not inhibitory: EDTA at 1-50 mM Actinomycetales bacterium
3.5.1.1 Zn2+ 10 mM, 34% residual activity Actinomycetales bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.0009
-
 L-asparagine pH not specified in the publication, temperature not specified in the publication Actinomycetales bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Actinomycetales bacterium KC573069 partial
-
3.5.1.1 Actinomycetales bacterium BkSoiiA KC573069 partial
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Actinomycetales bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 204
-
 pH not specified in the publication, temperature not specified in the publication Actinomycetales bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Actinomycetales bacterium L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Actinomycetales bacterium BkSoiiA L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 57000, SDS-PAGE Actinomycetales bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 10
-
-
 Actinomycetales bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.1 7 9
-
 Actinomycetales bacterium