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Literature summary extracted from

  • Das, S.; Roy Chowdhury, S.; Dey, S.; Sen, U.
    Structural and biochemical studies on Vibrio cholerae Hsp31 reveals a novel dimeric form and glutathione-independent glyoxalase activity (2017), PLoS ONE, 12, e0172629 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.124 cloned in pET28a+ vector, overexpressed in Escherichia coli BL21 (DE3) cells as N-terminus 6×His tagged fusion protein Vibrio cholerae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.124 crystals of VcHsp31 and its mutants are grown by hanging drop vapor diffusion method at different temperatures, from 18°C-25°C Vibrio cholerae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.124 C188A immeasurable glyoxalase activity Vibrio cholerae
3.5.1.124 E79A immeasurable glyoxalase activity Vibrio cholerae
3.5.1.124 H158A reduced amidopeptidase activity, glyoxalase activity is 53% compared to wild-type enzyme Vibrio cholerae
3.5.1.124 H189A immeasurable glyoxalase activity Vibrio cholerae
3.5.1.124 H76A immeasurable glyoxalase activity Vibrio cholerae
3.5.1.124 Y224A immeasurable glyoxalase activity Vibrio cholerae
3.5.1.124 Y224A reduced amidopeptidase activity Vibrio cholerae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.124 0.25084
-
 methylglyoxal pH 7.5, 37°C Vibrio cholerae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.124 methylglyoxal + H2O Vibrio cholerae
-
 (R)-lactate
-
 ?
3.5.1.124 methylglyoxal + H2O Vibrio cholerae O395
-
 (R)-lactate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.124 Vibrio cholerae A0A0H3AFW5
-
-
3.5.1.124 Vibrio cholerae O395 A0A0H3AFW5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.124
-
 Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.124 methylglyoxal + H2O
-
 Vibrio cholerae (R)-lactate
-
 ?
3.5.1.124 methylglyoxal + H2O the enzyme also shows aminopeptidase activity and protease activity Vibrio cholerae (R)-lactate
-
 ?
3.5.1.124 methylglyoxal + H2O
-
 Vibrio cholerae O395 (R)-lactate
-
 ?
3.5.1.124 methylglyoxal + H2O the enzyme also shows aminopeptidase activity and protease activity Vibrio cholerae O395 (R)-lactate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.124 dimer VcHsp31 acquires two (type-I and type-II) dimeric forms. Type-I dimer is similar to EcHsp31 where two VcHsp31 monomers associate in eclipsed manner through several intersubunit hydrogen bonds involving their P-domains. Type-II dimer is a dimeric organization, where some of the intersubunit hydrogen bonds are abrogated and each monomer swings out in the opposite directions centering at their P-domains, like twisting of wet cloth. Normal mode analysis (NMA) of type-I dimer shows similar movement of the individual monomers. Upon swinging, a dimeric surface of 400 A2, mostly hydrophobic in nature, is uncovered which might bind partially unfolded protein substrates. It is proposed that, in solution, VcHsp31 remains as an equilibrium mixture of both the dimers. With increase in temperature, transformation to type-II form having more exposed hydrophobic surface, occurs progressively accounting for the temperature dependent increase of chaperone activity of VcHsp31 Vibrio cholerae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.124 glutathione-independent glyoxalase
-
 Vibrio cholerae
3.5.1.124 Hsp31
-
 Vibrio cholerae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.124 37
-
 assay at Vibrio cholerae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.124 0.663
-
 methylglyoxal pH 7.5, 37°C Vibrio cholerae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.124 7.5
-
 assay at Vibrio cholerae

Expression

EC Number Organism Comment Expression
3.5.1.124 Vibrio cholerae stress-inducible enzyme up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.124 2.64
-
 methylglyoxal pH 7.5, 37°C Vibrio cholerae