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Literature summary extracted from

  • Jager, G.; Chen, P.; Bjork, G.R.
    Transfer RNA bound to mnmh protein is enriched with geranylated tRNA-a possible intermediate in its selenation (2016), PLoS One, 11, e0153488 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.9.1.3 G67E mutation in the rhodanese domain increases the intrinsic low geranylation activity of the wild type MnmH Salmonella enterica subsp. enterica serovar Typhimurium
2.9.1.3 G67E/K155A the mutant enzyme does not transfer in vitro a geranyl group from geranylpyrophosphate to tRNA Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.9.1.3 selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA + H2O Salmonella enterica subsp. enterica serovar Typhimurium the enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA 5-methylaminomethyl-2-selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.9.1.3 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZR88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.9.1.3
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.9.1.3 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-selenouridine34 in tRNA + phosphate
-
 ?
2.9.1.3 geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA + diphosphate
-
 ?
2.9.1.3 selenophosphate + 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol
-
 ?
2.9.1.3 selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA + H2O the enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.9.1.3 selU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.9.1.3 tRNA 2-selenouridine synthase
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 Salmonella enterica subsp. enterica serovar Typhimurium