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Literature summary extracted from

  • Anu, P.; Madanan, M.; Nair, A.; Nair, G.; Nair, G.; Sudhakaran, P.; Satheeshkumar, P.
    Heterologous expression, purification and characterization of an oligopeptidase A from the pathogen Leptospira interrogans (2018), Mol. Biotechnol., 60, 302-309 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.70 gene prlC, recombinant expression of N-terminally His-tagged enzyme in Escherichia ocli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Leptospira interrogans serovar Copenhageni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.70 Ca2+ about 75% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 Fe3+ about 90% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 Mg2+ about 75% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 Na+ about 90% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 Na-EDTA 50% inhibition at 10 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 phenylmethylsulfonyl fluoride PMSF, 80% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
3.4.24.70 trans-epoxysuccinyl L-leucylamido-(4-guanidino)butane E-64, 25% inhibition at 0.01 mM Leptospira interrogans serovar Copenhageni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.70 Cu2+ can substitute for Zn2+ Leptospira interrogans serovar Copenhageni
3.4.24.70 additional information OpdA is a metalloproteinase Leptospira interrogans serovar Copenhageni
3.4.24.70 Zn2+ required, activates Leptospira interrogans serovar Copenhageni

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.70 Leptospira interrogans serovar Copenhageni Q72SL8
-
-
3.4.24.70 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 Q72SL8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.70 recombinant His-tagged enzyme from Escherichia ocli strain BL21(DE3) by nickel affinity chromatography Leptospira interrogans serovar Copenhageni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.70 azocasein + H2O
-
 Leptospira interrogans serovar Copenhageni ?
-
 ?
3.4.24.70 azocasein + H2O
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.70 ? x * 71000, recombinant N-terminally His-tagged enzyme, SDS-PAGE Leptospira interrogans serovar Copenhageni

Synonyms

EC Number Synonyms Comment Organism
3.4.24.70 OpdA
-
 Leptospira interrogans serovar Copenhageni
3.4.24.70 prlC
-
 Leptospira interrogans serovar Copenhageni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.70 37
-
 recombinant enzyme Leptospira interrogans serovar Copenhageni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.70 7
-
 recombinant enzyme Leptospira interrogans serovar Copenhageni