EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.1 | expression in Escherichia coli | Pectobacterium carotovorum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Pectobacterium carotovorum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | recombinant enzyme, changes in the solubilization conditions of the L-asparaginase may increase by up to 25% its enzymatic activity | Pectobacterium carotovorum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | additional information | - |
L-asparaginase activity depends on buffers and ions and ranges from 386 I.U/mg in 50 mM sodium phosphate buffer pH 6.0, 375 mM NaCl up to 510 I.U/mg in 50 mM Tris-HCl pH 7.5, 500 mM NaCl | Pectobacterium carotovorum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 34 | - |
melting temperature, 50 mM sodium phosphate buffer, pH 6.0, 375 mM NaCl | Pectobacterium carotovorum |
3.5.1.1 | 39 | - |
melting temperature, 50 mM MES pH 6.0 | Pectobacterium carotovorum |
3.5.1.1 | 41.5 | - |
melting temperature, 50 mM Tris-HCl pH 9.0 | Pectobacterium carotovorum |