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Literature summary extracted from

  • Bibo-Verdugo, B.; Rojo-Arreola, L.; Navarrete-del-Toro, M.A.; Garcia-Carreno, F.
    A chymotrypsin from the digestive tract of California Spiny Lobster, Panulirus interruptus purification and biochemical characterization (2015), Mar. Biotechnol., 17, 416-427 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 acetone 10-50%, significantly reduces residual activity to about 80 % at all tested concentrations Panulirus interruptus
3.4.21.1 chymostatin 0.02 mM, complete loss of activity Panulirus interruptus
3.4.21.1 DMSO 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% Panulirus interruptus
3.4.21.1 additional information E-64 and pepstatin A have no effect on enzyme activity Panulirus interruptus
3.4.21.1 phenylmethylsulfonyl fluoride 1 mM, 95% loss of activity Panulirus interruptus
3.4.21.1 Propanol reduces the residual activity to about 80 % when tested at concentrations of 10 and 30%. Activity is not affected using 50% Panulirus interruptus
3.4.21.1 TLCK 1 mM, 10% loss of activity Panulirus interruptus
3.4.21.1 Toluene 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% Panulirus interruptus
3.4.21.1 TPCK 1 mM, 10% loss of activity Panulirus interruptus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.1 0.28
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.1 20100
-
 SDS-PAGE, enzyme is not heat-treated prior to electrophoresis Panulirus interruptus
3.4.21.1 28000
-
 SDS-PAGE, enzyme is incubated at 100°C for 10 min with and without DDT prior to electrophoresis Panulirus interruptus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Panulirus interruptus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.1
-
 Panulirus interruptus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 gastric juice
-
 Panulirus interruptus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.1 4.5
-
 substrate: succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide, pH 8.0, 25°C Panulirus interruptus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 Bovine serum albumin + H2O
-
 Panulirus interruptus ?
-
 ?
3.4.21.1 Collagen + H2O
-
 Panulirus interruptus ?
-
 ?
3.4.21.1 additional information no activity with benzoyl-Arg-p-nitroanilide Panulirus interruptus ?
-
 ?
3.4.21.1 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide + H2O
-
 Panulirus interruptus succinyl-L-Ala-Ala-Pro-L-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.1 ? x * 28000, SDS-PAGE Panulirus interruptus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.1 55
-
-
 Panulirus interruptus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.1 35 65 35°C: about 40% of maximal activity, 45°C: about 85% of maximal activity, 65°C: about 25% of maximal activity Panulirus interruptus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.1 25
-
 60 min, about 20% loss of activity Panulirus interruptus
3.4.21.1 35
-
 60 min, about 30% loss of activity Panulirus interruptus
3.4.21.1 45
-
 60 min, about 40% loss of activity Panulirus interruptus
3.4.21.1 55
-
 20 min, about 80% loss of activity. 40 min, complete loss of activity Panulirus interruptus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.1 1.07
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.1 7 8
-
 Panulirus interruptus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.1 6 9 pH 6.0: about 80% of maximal activity, pH 9.0: about 55% of maximal activity Panulirus interruptus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.1 1 2 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
3.4.21.1 3
-
 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
3.4.21.1 5
-
 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
3.4.21.1 8
-
 the enzyme conserves 100 % of its activity after 1 h Panulirus interruptus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.1 3.8
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus