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Literature summary extracted from

  • Liu, X.; Kopparapu, N.; Zheng, H.; Katrolia, P.; Deng, Y.; Zheng, X.
    Purification and characterization of a fibrinolytic enzyme from the food-grade fungus, Neurospora sitophila (2016), J. Mol. Catal. B, 134, 98-104 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.72 2-mercaptoethanol 146% activity at 0.5% (v/v) Neurospora sitophila
3.4.24.72 gelatin 121% activity at 1% (v/v) Neurospora sitophila
3.4.24.72 glycerol 129% activity at 30% (v/v) Neurospora sitophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 acetone complete inhibition at 10% (v/v) Neurospora sitophila
3.4.24.72 Aprotinin 82.3% residual activity at 5 mM Neurospora sitophila
3.4.24.72 bovine serum albumin complete inhibition at 1% (w/v) Neurospora sitophila
3.4.24.72 Cu2+ 61.2% residual activity at 5 mM Neurospora sitophila
3.4.24.72 cysteine 47.4% residual activity at 20 mM Neurospora sitophila
3.4.24.72 EDTA 13.5% residual activity at 5 mM Neurospora sitophila
3.4.24.72 Fe3+ complete inhibition at 5 mM Neurospora sitophila
3.4.24.72 L-lysine 38.5% residual activity at 5 mM Neurospora sitophila
3.4.24.72 oxidized glutathione complete inhibition at 5 mM Neurospora sitophila
3.4.24.72 pepstatin 71.5% residual activity at 2.5 mM Neurospora sitophila
3.4.24.72 peptone 61.5% residual activity at 1% (w/v) Neurospora sitophila
3.4.24.72 phenylmethane sulfonyl fluoride
-
 Neurospora sitophila
3.4.24.72 reduced glutathione complete inhibition at 5 mM Neurospora sitophila
3.4.24.72 SDS complete inhibition at 0.1% (w/v) Neurospora sitophila
3.4.24.72 Soybean trypsin inhibitor complete inhibition at 2 mM Neurospora sitophila
3.4.24.72 tosyl-phenylalanine chloromethyl ketone 76.2% residual activity at 5 mM Neurospora sitophila
3.4.24.72 Urea complete inhibition at 8 M Neurospora sitophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Ca2+ 390% activity at 5 mM Neurospora sitophila
3.4.24.72 Co2+ 114% activity at 5 mM Neurospora sitophila
3.4.24.72 Fe2+ 106% activity at 5 mM Neurospora sitophila
3.4.24.72 K+ 144% activity at 5 mM Neurospora sitophila
3.4.24.72 Mg2+ 208% activity at 5 mM Neurospora sitophila
3.4.24.72 Mn2+ 168% activity at 5 mM Neurospora sitophila
3.4.24.72 Na+ 219% activity at 5 mM Neurospora sitophila
3.4.24.72 Zn2+ 122% activity at 5 mM Neurospora sitophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.72 34000
-
 gel filtration Neurospora sitophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 fibrinogen + H2O Neurospora sitophila the alpha-chain of fibrinogen is degraded very quickly within 5 min, followed by beta-chain in 1 h whereas the gamma-chain is degraded in 2 h fibrin + ?
-
 ?
3.4.24.72 plasminogen + H2O Neurospora sitophila
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Neurospora sitophila
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.72 glycoprotein
-
 Neurospora sitophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 ammonium sulfate precipitation, octyl Sepharose column chromatography, SP Sepharose column chromatography, Sephadex G-25 gel filtration, and Superdex 75 gel filtration Neurospora sitophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 fibrinogen + H2O the alpha-chain of fibrinogen is degraded very quickly within 5 min, followed by beta-chain in 1 h whereas the gamma-chain is degraded in 2 h Neurospora sitophila fibrin + ?
-
 ?
3.4.24.72 plasminogen + H2O
-
 Neurospora sitophila plasmin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 monomer 1 * 34000, SDS-PAGE Neurospora sitophila

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 fibrinolytic protease
-
 Neurospora sitophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 41
-
-
 Neurospora sitophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.72 23 46 more than 50% activity between 23 and 46°C Neurospora sitophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.72 23 60 the enzyme is highly thermostable up to 52°C retaining more than 40% of its initial activity when incubated at 23-60°C for 1 h and 2 h Neurospora sitophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 7.6
-
-
 Neurospora sitophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.72 5.2 8.8 more than 60% activity between pH 5.2 and 8.8 Neurospora sitophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.72 5 11 the enzyme remains stable for 2 h at pH 5.0-11.0, retaining more than 80% activity Neurospora sitophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.72 Neurospora sitophila isoelectric focusing
-
 9.3